The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 185 kDa (predicted molecular weight: 171 kDa).
1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Is unsuitable for ICC,IHC-P or IP.
Atypical tyrosine-protein kinase that plays a central role in chromatin remodeling and acts as a transcription regulator. Involved in DNA damage response by phosphorylating 'Tyr-142' of histone H2AX (H2AXY142ph). H2AXY142ph plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. In the complex, it mediates the recruitment of the WICH complex to replication foci during DNA replication. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. In the WINAC complex, plays an essential role by targeting the complex to acetylated histones, an essential step for VDR-promoter association.
Ubiquitously expressed with high levels of expression in heart, brain, placenta, skeletal muscle and ovary.
Involvement in disease
Note=BAZ1B is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of BAZ1B may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
Bromodomain adjacent to zinc finger domain protein 1B antibody
hWALP 2 antibody
transcription factor WSTF antibody
Tyrosine-protein kinase BAZ1B antibody
WBRS 9 antibody
WBSC 10 antibody
Williams Beuren syndrome chromosome region 9 protein antibody
Williams syndrome transcription factor antibody
Williams-Beuren syndrome chromosomal region 10 protein antibody
Williams-Beuren syndrome chromosomal region 9 protein antibody
Western blot - Anti-WSTF antibody [EPR1703] (ab109439)
Predicted band size : 171 kDa
Lane 1: Wild type HAP1 whole cell lysate (40 µg) Lane 2: BAZ1B knockout HAP1 whole cell lysate (40 µg) Lane 3: HeLa whole cell lysate (40 µg) Lanes 1 - 3: Merged signal (red and green). Green - ab109439 observed at 171 kDa. Red - loading control, ab18058, observed at 130 kDa.
Ab109439 was shown to recognize BAZ1B in wild-type cells along with additional cross-reactive bands as signal was lost in BAZ1B knockout samples. Wild-type and BAZ1B knockout samples were subjected to SDS-PAGE. Ab109439 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Western blot - WSTF antibody [EPR1703] (ab109439)
All lanes : Anti-WSTF antibody [EPR1703] (ab109439) at 1/1000 dilution
Lane 1 : 293T cell lysates Lane 2 : HeLa cell lysates Lane 3 : HT-1080 cell lysates Lane 4 : PC-12 cell lysates Lane 5 : SH-SY5Y cell lysates