The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 44 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen.
Use a concentration of 3 µg/ml.
FunctionRegulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes. May be involved in mRNA splicing regulation.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the fl(2)d family.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.