1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is also possible using Tris-EDTA buffer (pH 8.0).
1/100 - 1/1000.
Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes. May be involved in mRNA splicing regulation.
Belongs to the fl(2)d family.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling WTAP using ab155655 at a 1/500 dilution (green). The slide was costained with an alpha Tubulin antibody (red) at a 1/2000 dilution.