Anti-WWOX antibody (ab137726)
Key features and details
- Rabbit polyclonal to WWOX
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-WWOX antibody
See all WWOX primary antibodies -
Description
Rabbit polyclonal to WWOX -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-186 of Human WWOX.
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Positive control
- 293T, HepG2, Molt4, Raji and Mouse brain cell lysates; A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137726 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 47 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 47 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Putative oxidoreductase. Acts as a tumor suppressor and plays a role in apoptosis. Required for normal bone development (By similarity). May function synergistically with p53/TP53 to control genotoxic stress-induced cell death. Plays a role in TGFB1 signaling and TGFB1-mediated cell death. May also play a role in tumor necrosis factor (TNF)-mediated cell death. Inhibits Wnt signaling, probably by sequestering DVL2 in the cytoplasm. -
Tissue specificity
Widely expressed. Strongly expressed in testis, prostate, and ovary. Overexpressed in cancer cell lines. Isoform 5 and isoform 6 may only be expressed in tumor cell lines. -
Involvement in disease
Note=Defects in WWOX may be involved in several cancer types. The gene spans the second most common chromosomal fragile site (FRA16D) which is frequently altered in cancers. Alteration of the expression and expression of some isoforms is associated with cancers. However, it is still unclear if alteration of WWOX is directly implicated in cancerogenesis or if it corresponds to a secondary effect.
Defects in WWOX may be a cause of esophageal cancer (ESCR) [MIM:133239]. -
Sequence similarities
Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Contains 2 WW domains. -
Domain
The WW 1 domain mediates interaction with TP53, and probably TP73, TFAP2C, LITAF and WBP1. -
Post-translational
modificationsPhosphorylated upon genotoxic stress. Phosphorylation of Tyr-33 regulates interaction with TP53, TP73 and MAPK8. May also regulate proapoptotic activity. Phosphorylation by TNK2 is associated with polyubiquitination and degradation.
Ubiquitinated when phosphorylated by TNK2, leading to its degradation. -
Cellular localization
Cytoplasm. Nucleus. Mitochondrion. Golgi apparatus. Partially localizes to the mitochondria. Translocates to the nucleus upon genotoxic stress or TNF stimulation (By similarity). Translocates to the nucleus in response to TGFB1. Isoform 5 and isoform 6 may localize in the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 618792 Cow
- Entrez Gene: 51741 Human
- Entrez Gene: 80707 Mouse
- Entrez Gene: 292041 Rat
- Omim: 605131 Human
- SwissProt: Q9NZC7 Human
- SwissProt: Q91WL8 Mouse
- Unigene: 461453 Human
see all -
Alternative names
- 5330426P09Rik antibody
- 9030416C10Rik antibody
- Aberrant WW domain-containing oxidoreductase antibody
see all
Images
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Anti-WWOX antibody (ab137726) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 47 kDa
12% SDS PAGE -
Anti-WWOX antibody (ab137726) at 1/10000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 47 kDa
12% SDS PAGE -
Immunofluorescent analysis of paraformaldehyde-fixed A549 cells labelling WWOX with ab137726 at 1/200 dilution. Lower panel shows the image merged with DNA probe.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab137726 has been referenced in 5 publications.
- Xu D et al. LncRNA WWOX-AS1 sponges miR-20b-5p in hepatocellular carcinoma and represses its progression by upregulating WWOX. Cancer Biol Ther 21:927-936 (2020). PubMed: 32931356
- Janczar S et al. WWOX sensitises ovarian cancer cells to paclitaxel via modulation of the ER stress response. Cell Death Dis 8:e2955 (2017). WB . PubMed: 28749468
- Zhou Y et al. Adenovirus-delivered wwox inhibited lung cancer growth in vivo in a mouse model. Cancer Gene Ther 23:1-6 (2016). PubMed: 26516139
- Gao K et al. Deregulated WWOX is involved in a negative feedback loop with microRNA-214-3p in osteosarcoma. Int J Mol Med N/A:N/A (2016). WB . PubMed: 27840941
- Abu-Remaileh M & Aqeilan RI Tumor suppressor WWOX regulates glucose metabolism via HIF1a modulation. Cell Death Differ N/A:N/A (2014). PubMed: 25012504