Overview

  • Product nameAnti-WWOX antibody
    See all WWOX primary antibodies
  • Description
    Mouse polyclonal to WWOX
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein corresponding to amino acids 1-189 of Human WWOX (NP_570607.1).

  • Positive control
    • 293 cell lysate, WWOX transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab89128 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa.

Target

  • FunctionPutative oxidoreductase. Acts as a tumor suppressor and plays a role in apoptosis. Required for normal bone development (By similarity). May function synergistically with p53/TP53 to control genotoxic stress-induced cell death. Plays a role in TGFB1 signaling and TGFB1-mediated cell death. May also play a role in tumor necrosis factor (TNF)-mediated cell death. Inhibits Wnt signaling, probably by sequestering DVL2 in the cytoplasm.
  • Tissue specificityWidely expressed. Strongly expressed in testis, prostate, and ovary. Overexpressed in cancer cell lines. Isoform 5 and isoform 6 may only be expressed in tumor cell lines.
  • Involvement in diseaseNote=Defects in WWOX may be involved in several cancer types. The gene spans the second most common chromosomal fragile site (FRA16D) which is frequently altered in cancers. Alteration of the expression and expression of some isoforms is associated with cancers. However, it is still unclear if alteration of WWOX is directly implicated in cancerogenesis or if it corresponds to a secondary effect.
    Defects in WWOX may be a cause of esophageal cancer (ESCR) [MIM:133239].
  • Sequence similaritiesBelongs to the short-chain dehydrogenases/reductases (SDR) family.
    Contains 2 WW domains.
  • DomainThe WW 1 domain mediates interaction with TP53, and probably TP73, TFAP2C, LITAF and WBP1.
  • Post-translational
    modifications
    Phosphorylated upon genotoxic stress. Phosphorylation of Tyr-33 regulates interaction with TP53, TP73 and MAPK8. May also regulate proapoptotic activity. Phosphorylation by TNK2 is associated with polyubiquitination and degradation.
    Ubiquitinated when phosphorylated by TNK2, leading to its degradation.
  • Cellular localizationCytoplasm. Nucleus. Mitochondrion. Golgi apparatus. Partially localizes to the mitochondria. Translocates to the nucleus upon genotoxic stress or TNF stimulation (By similarity). Translocates to the nucleus in response to TGFB1. Isoform 5 and isoform 6 may localize in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5330426P09Rik antibody
    • 9030416C10Rik antibody
    • Aberrant WW domain-containing oxidoreductase antibody
    • D16S432E antibody
    • EC 1.1.1.- antibody
    • EIEE28 antibody
    • FOR antibody
    • FRA16D antibody
    • Fragile site FRA16D oxidoreductase antibody
    • Fragile site FRA16D Oxireductase antibody
    • HHCMA56 antibody
    • MGC55975 antibody
    • PRO0128 antibody
    • Putative oxidoreductase antibody
    • SCAR12 antibody
    • SDR41C1 antibody
    • Short chain dehydrogenase/reductase family 41C, member 1 antibody
    • WOX1 antibody
    • WW domain containing oxidoreductase antibody
    • WW domain-containing oxidoreductase antibody
    • WW domain-containing protein WWOX antibody
    • wwox antibody
    • WWOX_HUMAN antibody
    • zgc:55975 antibody
    see all

Anti-WWOX antibody images

  • Anti-WWOX antibody (ab89128) at 1 µg/ml + 293 cell lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 47 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-WWOX antibody (ab89128) at 1 µg/ml

    Lane 1 : WWOX transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Developed using the ECL technique

    Predicted band size : 47 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)

References for Anti-WWOX antibody (ab89128)

ab89128 has not yet been referenced specifically in any publications.

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