Overview

  • Product name
    Anti-WWOX antibody - N-terminal
    See all WWOX primary antibodies
  • Description
    Rabbit polyclonal to WWOX - N-terminal
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment within Human WWOX aa 1-186 (N terminal). The exact sequence is proprietary. (NP_057457.1)
    Database link: Q9NZC7

  • Positive control
    • Human placenta tissue; A549 cells; Raji whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab189410 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 47 kDa.
IHC-P Use a concentration of 10 µg/ml.
ICC/IF 1/100 - 1/200.

Target

  • Function
    Putative oxidoreductase. Acts as a tumor suppressor and plays a role in apoptosis. Required for normal bone development (By similarity). May function synergistically with p53/TP53 to control genotoxic stress-induced cell death. Plays a role in TGFB1 signaling and TGFB1-mediated cell death. May also play a role in tumor necrosis factor (TNF)-mediated cell death. Inhibits Wnt signaling, probably by sequestering DVL2 in the cytoplasm.
  • Tissue specificity
    Widely expressed. Strongly expressed in testis, prostate, and ovary. Overexpressed in cancer cell lines. Isoform 5 and isoform 6 may only be expressed in tumor cell lines.
  • Involvement in disease
    Note=Defects in WWOX may be involved in several cancer types. The gene spans the second most common chromosomal fragile site (FRA16D) which is frequently altered in cancers. Alteration of the expression and expression of some isoforms is associated with cancers. However, it is still unclear if alteration of WWOX is directly implicated in cancerogenesis or if it corresponds to a secondary effect.
    Defects in WWOX may be a cause of esophageal cancer (ESCR) [MIM:133239].
  • Sequence similarities
    Belongs to the short-chain dehydrogenases/reductases (SDR) family.
    Contains 2 WW domains.
  • Domain
    The WW 1 domain mediates interaction with TP53, and probably TP73, TFAP2C, LITAF and WBP1.
  • Post-translational
    modifications
    Phosphorylated upon genotoxic stress. Phosphorylation of Tyr-33 regulates interaction with TP53, TP73 and MAPK8. May also regulate proapoptotic activity. Phosphorylation by TNK2 is associated with polyubiquitination and degradation.
    Ubiquitinated when phosphorylated by TNK2, leading to its degradation.
  • Cellular localization
    Cytoplasm. Nucleus. Mitochondrion. Golgi apparatus. Partially localizes to the mitochondria. Translocates to the nucleus upon genotoxic stress or TNF stimulation (By similarity). Translocates to the nucleus in response to TGFB1. Isoform 5 and isoform 6 may localize in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5330426P09Rik antibody
    • 9030416C10Rik antibody
    • Aberrant WW domain-containing oxidoreductase antibody
    • D16S432E antibody
    • EC 1.1.1.- antibody
    • EIEE28 antibody
    • FOR antibody
    • FRA16D antibody
    • Fragile site FRA16D oxidoreductase antibody
    • Fragile site FRA16D Oxireductase antibody
    • HHCMA56 antibody
    • MGC55975 antibody
    • PRO0128 antibody
    • Putative oxidoreductase antibody
    • SCAR12 antibody
    • SDR41C1 antibody
    • Short chain dehydrogenase/reductase family 41C, member 1 antibody
    • WOX1 antibody
    • WW domain containing oxidoreductase antibody
    • WW domain-containing oxidoreductase antibody
    • WW domain-containing protein WWOX antibody
    • wwox antibody
    • WWOX_HUMAN antibody
    • zgc:55975 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue labeling WWOX3 with ab189410 at 10 µg/ml.

  • Immunofluorescent analysis of paraformaldehyde-fixed A549 cells labeling WWOX3 with ab189410 at 1/200 dilution. Lower panel merged with DNA probe.

  • Anti-WWOX antibody - N-terminal (ab189410) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 47 kDa

    12% SDS PAGE

References

This product has been referenced in:
  • Luo L  et al. Reduced expression of the WW domain-containing oxidoreductase in human hematopoietic malignancies. Oncol Lett 11:4083-4088 (2016). Read more (PubMed: 27313745) »
  • Xie B  et al. ACK1 promotes hepatocellular carcinoma progression via downregulating WWOX and activating AKT signaling. Int J Oncol 46:2057-66 (2015). Read more (PubMed: 25738261) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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