Anti-WWP1 antibody (ab227213)
Key features and details
- Rabbit polyclonal to WWP1
- Suitable for: IHC-P
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-WWP1 antibody
See all WWP1 primary antibodies -
Description
Rabbit polyclonal to WWP1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Chicken, Cow, Human, Rhesus monkey -
Immunogen
Recombinant fragment within Human WWP1 (internal sequence). The exact sequence is proprietary.
Database link: Q9H0M0 -
Positive control
- IHC-P: Mouse liver and stomach tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227213 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/100 - 1/1000.
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Notes |
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IHC-P
1/100 - 1/1000. |
Target
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Relevance
WWP1 is an E3 ubiquitin ligase and belongs to a family of NEDD4-like proteins. WWP1 contains 4 tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. The HECT domain of WWP1 has been implicated in regulating the localization and stability of p53 – inhibition of WWP1 results in a decrease in p53 expression, whilst WWP1 mediated stabilization of p53 appears to be associated with an accumulation of cytoplasmic p53. WWP1 also negatively regulates the TGF beta tumor suppressor pathway by inactivating its molecular components (SMAD2, SMAD4 and TGFbetaR1). WWP1 has been implicated in both breast and prostate cancers. -
Cellular localization
Cell Membrane, Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 420212 Chicken
- Entrez Gene: 513789 Cow
- Entrez Gene: 11059 Human
- Entrez Gene: 107568 Mouse
- Entrez Gene: 297930 Rat
- Entrez Gene: 694098 Rhesus monkey
- Omim: 602307 Human
- SwissProt: Q9H0M0 Human
see all -
Alternative names
- AIP 5 antibody
- AIP5 antibody
- Atropin-1-interacting protein 5 antibody
see all
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab227213 has not yet been referenced specifically in any publications.