Overview

  • Product nameAnti-Wwp2 antibody
    See all Wwp2 primary antibodies
  • Description
    Rabbit polyclonal to Wwp2
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 200 and 250 of Human Wwp2 (NP_008945.2).

  • Positive control
    • HeLa, 293T and mouse NIH3T3 whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab103527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 99 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • RelevanceWwp2 is a HECT domain containing E3 ubiqutin protein ligase. Wwp2 specifically interacts with Rpb1 and is responsible for targeting it for ubiquitination.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • AIP2 antibody
    • Atrophin 1 interacting protein 2 antibody
    • NEDD4 like E3 ubiquitin protein ligase antibody
    • WW domain-containing protein 2 antibody
    • Wwp 2 antibody
    see all

Anti-Wwp2 antibody images

  • All lanes : Anti-Wwp2 antibody (ab103527) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T at 50 µg
    Lane 5 : NIH3T3 at 50 µg

    Developed using the ECL technique

    Predicted band size : 99 kDa


    Exposure time : 3 minutes
  • Detection of Wwp2 by Western Blot of Immunprecipitate.
    Lane 1: ab103527 at 1µg/ml staining Wwp2 in HeLa whole cell lysate immunoprecipitated using ab103527 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Lane 2: Control IgG
    Detection: Chemiluminescence with exposure time of 30 seconds.

References for Anti-Wwp2 antibody (ab103527)

ab103527 has not yet been referenced specifically in any publications.

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