Customer reviews and FAQs

Overview

  • Product name
    Anti-Wwp2 antibody
    See all Wwp2 primary antibodies
  • Description
    Rabbit polyclonal to Wwp2
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • Human kidney tissue, Jurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab60130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 99, 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:312500.

Target

Anti-Wwp2 antibody images

  • Western blot - Wwp2 antibody (ab60130)
    Western blot - Wwp2 antibody (ab60130)
    Anti-Wwp2 antibody (ab60130) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/ 50,000 - 1/100,000 dilution.

    Predicted band size : 99, 47 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wwp2 antibody (ab60130)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wwp2 antibody (ab60130)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Wwp2 with ab60130 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

References for Anti-Wwp2 antibody (ab60130)

ab60130 has not yet been referenced specifically in any publications.

Publishing research using ab60130? Please let us know so that we can cite the reference in this datasheet.

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