- Datasheet
- Specific References
- Protocols
Overview
- Product nameAnti-Wwp2 antibody
See all Wwp2 primary antibodies - DescriptionRabbit polyclonal to Wwp2
- Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
- Immunogen
Synthetic peptide (Human)
- Positive control
- Human kidney tissue, Jurkat cell lysate.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... - PurityImmunogen affinity purified
- ClonalityPolyclonal
- IsotypeIgG
- Research areas
Associated products
- Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab60130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 99, 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. | |
| IHC-P | Use a concentration of 4 - 8 µg/ml. | |
| ELISA | Use at an assay dependent concentration. Titre using peptide based assay: 1:312500. |
Target
- RelevanceWwp2 is a HECT domain containing E3 ubiqutin protein ligase. Wwp2 specifically interacts with Rpb1 and is responsible for targeting it for ubiquitination.
- Cellular localizationNuclear
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Database links
- Entrez Gene: 479672 Dog
- Entrez Gene: 11060 Human
- Entrez Gene: 66894 Mouse
- Entrez Gene: 291999 Rat
- Omim: 602308 Human
- SwissProt: O00308 Human
- SwissProt: Q9DBH0 Mouse
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Alternative names
- AIP2 antibody
- Atrophin 1 interacting protein 2 antibody
- NEDD4 like E3 ubiquitin protein ligase antibody
see all
Anti-Wwp2 antibody images
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Western blot - Wwp2 antibody (ab60130)Anti-Wwp2 antibody (ab60130) at 1 µg/ml + Jurkat cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/ 50,000 - 1/100,000 dilution.
Predicted band size : 99, 47 kDa
Observed band size : 52 kDa (why is the actual band size different from the predicted?) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wwp2 antibody (ab60130)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Wwp2 with ab60130 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.
References for Anti-Wwp2 antibody (ab60130)
ab60130 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"