- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Wwp2 antibody
See all Wwp2 primary antibodies -
DescriptionRabbit polyclonal to Wwp2
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
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Immunogen
Synthetic peptide corresponding to Human Wwp2 aa 451-500 (internal sequence).
Sequence:EMKYTSEGVRYFVDHNTRTTTFKDPRPGFESGTKQGSPGAYDRSFRWKYH
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Positive control
- Human kidney tissue, Jurkat cell lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab60130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 99, 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. | |
| IHC-P | Use a concentration of 4 - 8 µg/ml. | |
| ELISA | Use at an assay dependent concentration. Titre using peptide based assay: 1:312500. |
Target
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RelevanceWwp2 is a HECT domain containing E3 ubiqutin protein ligase. Wwp2 specifically interacts with Rpb1 and is responsible for targeting it for ubiquitination.
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Cellular localizationNuclear
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Database links
- Entrez Gene: 479672 Dog
- Entrez Gene: 11060 Human
- Entrez Gene: 66894 Mouse
- Entrez Gene: 291999 Rat
- Omim: 602308 Human
- SwissProt: O00308 Human
- SwissProt: Q9DBH0 Mouse
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Alternative names
- AIP2 antibody
- Atrophin 1 interacting protein 2 antibody
- NEDD4 like E3 ubiquitin protein ligase antibody
see all
Images
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Western blot - Wwp2 antibody (ab60130)Anti-Wwp2 antibody (ab60130) at 1 µg/ml + Jurkat cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/ 50,000 - 1/100,000 dilution.
Predicted band size: 99, 47 kDa
Observed band size: 52 kDa (why is the actual band size different from the predicted?) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wwp2 antibody (ab60130)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Wwp2 with ab60130 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.
References
ab60130 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"