Synthetic peptide corresponding to Human Wwp2 aa 451-500 (internal sequence).
Sequence:
EMKYTSEGVRYFVDHNTRTTTFKDPRPGFESGTKQGSPGAYDRSFRWKYH
Our Abpromise guarantee covers the use of ab60130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 99, 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. | |
IHC-P | Use a concentration of 4 - 8 µg/ml. | |
ELISA | Use at an assay dependent concentration. Titre using peptide based assay: 1:312500. |
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Wwp2 with ab60130 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.
ab60130 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"