Anti-xCT antibody (ab111822)

Overview

  • Product nameAnti-xCT antibody
    See all xCT primary antibodies
  • Description
    Goat polyclonal to xCT
  • Tested applicationsELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Pig, Orangutan
  • Immunogen

    Synthetic peptide:

    KGQTQNFKDAFSGRD

    by a Cysteine residue linker, corresponding to internal sequence amino acids 215-229 of Human xCT.

  • Positive control
    • Human Kidney and Placenta tissues; Human Spleen lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab111822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/128000.
WB Use a concentration of 0.02 - 0.06 µg/ml. Predicted molecular weight: 55 kDa.
IHC-P Use a concentration of 3.75 µg/ml.

Target

  • FunctionSodium-independent, high-affinity exchange of anionic amino acids with high specificity for anionic form of cystine and glutamate.
  • Sequence similaritiesBelongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Amino acid transport system xc xCT antibody antibody
    • Amino acid transport system xc- antibody
    • Calcium channel blocker resistance protein CCBR1 antibody
    • Calcium channel blocker resistance protein CCBR1 antibody antibody
    • CCBR1 antibody
    • Cysteine/glutamate transporter antibody antibody
    • Cystine/glutamate transporter antibody
    • OTTHUMP00000164578 antibody
    • SLC7A11 antibody
    • Solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11 antibody
    • solute carrier family 7 antibody
    • Solute carrier family 7 member 11 antibody
    • Solute carrier family 7, (cationic amino acid transporter, y+ system) member 11 antibody
    • SYSTEM Xc(-) TRANSPORTER-RELATED PROTEIN antibody
    • xCT antibody
    • XCT_HUMAN antibody
    see all

Anti-xCT antibody images

  • ab111822 at 3.75 µg/ml staining xCT in Formalin-Fixed, Paraffin-Embedded Human Kidney tissue by Immunohistochemistry.
  • ab111822 at 3.75 µg/ml staining xCT in Formalin-Fixed, Paraffin-Embedded Human Placenta tissue by Immunohistochemistry.
  • Anti-xCT antibody (ab111822) at 0.02 µg/ml (for one hour) + Human Spleen lysate in RIPA buffer at 35 µg
    developed using the ECL technique

    Predicted band size : 55 kDa

References for Anti-xCT antibody (ab111822)

ab111822 has not yet been referenced specifically in any publications.

Product Wall

We have added product ab178004, xCT peptide, as a blocking peptide to be used with ab111822. Here is a link: http://www.abcam.com/xct-peptide-ab178004.html.

Thank you for your reply.

I have added the code to your Abreview. This new code is now active and can be used immediatlely. I am sorry for any issues which had arisen during this process.

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Application Flow Cytometry
Sample Human Cell (liver hepatocellular cells HepG2)
Specification liver hepatocellular cells HepG2
Preparation Cell harvesting/tissue preparation method: trypsinized and resuspended in PBS+1% serum+0.1% NaN3
Sample buffer: PBS+1% serum+0.1% NaN3
Fixation did not fix
Permeabilization No
Gating Strategy ungated cell population
Username

Yuzhe

Verified customer

Submitted Sep 05 2012

Thank you for contacting me regarding this. I have alerted our technical team to the problems which you have had when trying to add this information to your Abreview. As this issue has arisen, I have thought it best to create a whole new code for yo...

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Thank you for your reply.

Yes, please go ahead and place the first order as normal, you do not need the discount code for this order. Once you have tested the antibody and submitted the Abreview, then please place the second order and this ti...

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DISCOUNT CODE: **********
Expiration date: **********

I am very pleased to hear you would like to accept our offer and test ab111822 in Flow Cytometry. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To rede...

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Thank you for contacting us. You are correct, ab111822 is raised against an extracellular region of xCT. As such you should not have to permeablize your cells to use this product. I hope that this information is helpful. Please let me know if y...

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Thank you for your response and for the additional details which you have provided.

You are correct as this antibody will only recognize the intracellular epitope of xCT. As such you would need to permeablize your cells to allow entry of the...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"