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Western blot - xCT antibody (ab37183)
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Product Name
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xCT antibody
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See all xCT antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to xCT
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Immunogen
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Synthetic peptide: MVRKPVVSTI SKGGYLQGN V NGRLPSLG NK EPPGQEK VQL KRKVTL LRGV, corresponding to N terminal amino acids 1/50 of Mouse xCT
BLAST 'MVRKPVVSTI SKGGYLQGN V NGRLPSLG NK EPPGQEK VQL KRKVTL ' with  or 
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Reacts with
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Hu
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Does not react with
(species key)
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Ms
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Specificity
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This antibody is specific for XCT.
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 µg/ml(ECL). Predicted molecular weight: 55 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
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human Stomach tissue lysate.
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Cellular localization
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Membrane; multi-pass membrane protein
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Research areas
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Cell Biology >> Other Antibodies >> Oxidative Stress
Signal Transduction >> Metabolism >> Plasma Membrane >> Channels
Signal Transduction >> Metabolism >> Amino Acids
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Relevance
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Mammalian amino acid transport systems consist of a large variety of transporters. The amino acid transporter, cystine/glutamic acid transporter xCT, for system xc has been proposed to be responsible for the cystine transport through the plasma membrane. System xc mediates an amino acid exchange and prefers cystine and glutamate as its substrates. The transporter designated as xCT requires 4F2 heavy chain (4F2hc:CD98) for its functional expression, and belongs to the family of amino acid transporters that associates with the type II membrane glycoproteins such as 4F2hc. In brain, it has been proposed that system xc is up-regulated in glial cells upon oxidative stress and plays an essential roles to protect neurons against oxidative stress. Recent studies have implicated xCT as a gateway that Kaposi's sarcoma herpesvirus (KSHV) uses to enter cells.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: Tris glycine, 150mM Sodium chloride Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.400 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this xCT antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for xCT antibody images, references, products related to ab37183 and other tools.
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xCT antibody images:
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 Western blot - xCT antibody (ab37183)
xCT antibody (ab37183) (total human stomach lysate)
developed using the ECL technique
Predicted band size : 55 kDa
Observed band size : 55 kDa
Exposure time : 15 minutes
Lysate loading concentration: 50 µg
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Search PubMed (MEDLINE) for references to xCT
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xCT antibody - more information
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Customer reviews (feedback) regarding xCT antibody |
Customer FAQs regarding xCT antibody |
Protocols for xCT antibody |
Price and availability of products related to xCT antibody |
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xCT antibody - related products:
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xCT antibody - other tools:
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Contact Abcam with a Technical Enquiry about xCT antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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