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Products:Signal Transduction >> Metabolism >> Amino Acids
Overview
Product name
Anti-xCT antibody
See all xCT products (7) ...
Description
Rabbit polyclonal to xCT
Specificity
This antibody is specific for XCT.
Tested applications
WB, Flow Cyt, IHC-FoFrmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide: MVRKPVVSTI SKGGYLQGNV NGRLPSLGNK EPPGQEKVQL , corresponding to N terminal amino acids 1/50 of Human xCT
MVRKPVVSTI SKGGYLQGNV NGRLPSLGNK EPPGQEKVQL KRKVTLLRGV
Positive control
human and mouse Stomach tissue lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: Tris glycine, 150mM Sodium chloride
Concentration
Concentration information loading...
Purity
Protein A purified
Purification notes
Affinity purified antibody.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Other Antibodies >> Oxidative Stress
Signal Transduction >> Metabolism >> Plasma Membrane >> Channels
Signal Transduction >> Metabolism >> Amino Acids
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Western blot - xCT antibody (ab37185)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab37185 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 2 µg/ml.Predicted molecular weight: 55 kDa.( (ECL). )
Flow Cyt: Use at an assay dependent dilution.
IHC-FoFr: Use at an assay dependent dilution. (PubMed: 21639880)
Target
Function
Sodium-independent, high-affinity exchange of anionic amino acids with high specificity for anionic form of cystine and glutamate.
Sequence similarities
Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.
Cellular localization
Membrane.
Target information above from: UniProt accessionQ9UPY5
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Amino acid transport system xc xCT antibody antibody
- Amino acid transport system xc- antibody
- Calcium channel blocker resistance protein CCBR1 antibody
see all
Database links
- Entrez Gene: 23657 Human
- Entrez Gene: 26570 Mouse
- Omim: 607933 Human
- SwissProt: Q9UPY5 Human
- SwissProt: Q9WTR6 Mouse
- Unigene: 390594 Human
- Unigene: 260988 Mouse
Anti-xCT antibody images:
Western blot - xCT antibody (ab37185)
All lanes : Anti-xCT antibody (ab37185) at 0.5 µg/ml
Lane 1 : Total human stomach lysate.
Lane 2 : Total mouse stomach lysate.
developed using the ECL technique
Predicted band size : 55 kDa
Observed band size : 55 kDa
Exposure time : 1 minute
Lysate loading concentration: 50µg
References for Anti-xCT antibody (ab37185)
This product has been referenced in:
- Pampliega Oet al. Increased expression of cystine/glutamate antiporter in multiple sclerosis. J Neuroinflammation 8:63 (2011). WB, Flow Cyt, IHC-FoFr; Human, Mouse, Rat.Read more (PubMed: 21639880) »
- Srivastava MKet al. Myeloid-derived suppressor cells inhibit T-cell activation by depleting cystine and cysteine. Cancer Res 70:68-77 (2010). Flow Cyt; Mouse.Read more (PubMed: 20028852) »
See all 3 publications for this product
Publishing research using ab37185? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"