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Products:Signal Transduction >> Metabolism >> Amino Acids
Overview
Product name
Anti-xCT antibody
See all xCT products (7) ...
Description
Goat polyclonal to xCT
Tested applications
Cross reactivity
Reacts with
Rat, Human
Predicted to work with
Mouse, Dog, Pig, Orangutan
Immunogen
Synthetic peptide: C-KGQTQNFKDAFSGRD, corresponding to internal sequence amino acids 215-229 of Human xCT
C-KGQTQNFK DAFSGRD
Positive control
Human Spleen lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Other Antibodies >> Oxidative Stress
Signal Transduction >> Metabolism >> Plasma Membrane >> Channels
Signal Transduction >> Metabolism >> Amino Acids
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Western blot - xCT antibody (ab60171)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab60171 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.02 - 0.06 µg/ml.Detects a band of approximately 52 kDa (predicted molecular weight: 55 kDa).
Target
Function
Sodium-independent, high-affinity exchange of anionic amino acids with high specificity for anionic form of cystine and glutamate.
Sequence similarities
Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.
Cellular localization
Membrane.
Target information above from: UniProt accessionQ9UPY5
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Amino acid transport system xc xCT antibody antibody
- Amino acid transport system xc- antibody
- Calcium channel blocker resistance protein CCBR1 antibody
see all
Database links
- Entrez Gene: 483821 Dog
- Entrez Gene: 23657 Human
- Entrez Gene: 26570 Mouse
- Entrez Gene: 100172659 Orangutan
- Entrez Gene: 100049683 Pig
- Entrez Gene: 310392 Rat
- Omim: 607933 Human
- SwissProt: Q9UPY5 Human
see all
Anti-xCT antibody images:
Western blot - xCT antibody (ab60171)
Anti-xCT antibody (ab60171) at 0.02 µg/ml + Human Spleen lysate (35µg protein in RIPA buffer)
Predicted band size : 55 kDa
Observed band size : 52 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence.
References for Anti-xCT antibody (ab60171)
This product has been referenced in:
- Chintala Set al. Downregulation of Cystine Transporter x(c) by Irinotecan in human head and neck cancer FaDu xenografts. Chemotherapy 56:223-33 (2010).Read more (PubMed: 20551639) »
- Olm Eet al. Extracellular thiol-assisted selenium uptake dependent on the x(c)- cystine transporter explains the cancer-specific cytotoxicity of selenite. Proc Natl Acad Sci U S A 106:11400-5 (2009). WB; Human.Read more (PubMed: 19549867) »
See all 2 publications for this product
Publishing research using ab60171? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"