• Product nameAnti-XAF1 antibody
    See all XAF1 primary antibodies
  • Description
    Rabbit polyclonal to XAF1
  • Specificityab25889 recognises XAF-1.
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human) - corresponding to 16 amino acids near the middle of human XAF-1.

  • Positive control
    • Human spleen cell lysate.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab25889 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 34 kDa.Can be blocked with Human XAF1 peptide (ab39908).


  • FunctionSeems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti-caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha-induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondra, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells.
  • Tissue specificityWidely expressed. Expression is frequently down-regulated in cancer cell lines. Isoform 5 is widely expressed. Expressed in placenta (at protein level).
  • Sequence similaritiesContains 1 TRAF-type zinc finger.
  • Developmental stageSelectively expressed in third trimester placenta.
  • Cellular localizationNucleus and Cytoplasm. Nucleus. Mitochondrion. Found in the cytoplasm and nucleus of placental syncytiotrophoblasts. Translocates to mitochondria upon TNF-alpha treatment.
  • Information by UniProt
  • Database links
  • Alternative names
    • BIRC 4 binding protein antibody
    • BIRC4 binding protein antibody
    • BIRC4-binding protein antibody
    • BIRC4BP antibody
    • HSXIAPAF 1 antibody
    • HSXIAPAF1 antibody
    • XAF 1 antibody
    • XAF1 antibody
    • XAF1_HUMAN antibody
    • XIAP associated factor 1 antibody
    • XIAP-associated factor 1 antibody
    • XIAPAF1 antibody
    see all

Anti-XAF1 antibody images

  • Lane 1 : Anti-XAF1 antibody (ab25889) at 1 µg/ml
    Lane 2 : Anti-XAF1 antibody (ab25889) at 2 µg/ml
    Lane 3 : Anti-XAF1 antibody (ab25889) at 4 µg/ml

    Lane 1 : Human spleen cell lysate.
    Lane 2 : Human spleen cell lysate.
    Lane 3 : Human spleen cell lysate.

    Predicted band size : 34 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

References for Anti-XAF1 antibody (ab25889)

This product has been referenced in:
  • Desplanques G  et al. Impact of XIAP protein levels on the survival of myeloma cells. Haematologica 94:87-93 (2009). WB ; Human . Read more (PubMed: 19001278) »
  • Bai Y  et al. Interferon-gamma induces XIAP-associated factor-1 and NOXA expression and potentiates human vascular smooth muscle cell apoptosis by STAT3 activation. J Biol Chem : (2008). WB ; Human . Read more (PubMed: 18192275) »

See all 2 Publications for this product

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