Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab27477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Tissue specificity
    In normal tissues, highly expressed in testis. Expressed also in many different types of cancers: highly expressed in breast cancer, prostate cancer and many types of lung cancers, including squamous cell carcinoma, small cell carcinoma, non-small cell carcinoma, and adenocarcinoma, as well as in Ewing's cell lines, in some Ewing's sarcoma patient samples, and in one of one alveolar rhabdomyosarcoma patient sample.
  • Sequence similarities
    Belongs to the GAGE family.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cancer/testis antigen 12.1 antibody
    • CT12.1 antibody
    • G antigen family D 2 protein antibody
    • G antigen family D member 2 antibody
    • GAGD2_HUMAN antibody
    • GAGED2 antibody
    • Protein XAGE-1 antibody
    • X antigen family, member 1 antibody
    • XAGE1A antibody
    • XAGE1B antibody
    • XAGE1C antibody
    • XAGE1D antibody
    • XAGE1E antibody
    see all

Images

  • ab27477 (3.8µg/ml) staining of paraffin embedded Human Testis shows polar cytoplasm staining in primary spermatocytes. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

References

ab27477 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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