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Xanthine Oxidase Activity Assay Kit (ab102522) is an easy and sensitive assay to determine xanthine oxidase (XO) activity in variety of samples. In the assay, XO present in the sample oxidizes xanthine to hydrogen peroxide (H2O2) which reacts stoichiometrically with OxiRed Probe to generate color (at OD = 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the color or fluorescence intensity is proportional to XO content, the XO activity can be accurately measured. The kit detects 1-100 mU xanthine oxidase in 100 µl reaction volume.
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Xanthine oxidase (XO, EC 220.127.116.11 ) is present in appreciable amounts in the liver and jejunum in healthy individuals. However, in various liver disorders, XO is released into circulation. Therefore, determination of serum XO level serves as a sensitive indicator of acute liver damage such as jaundice.
|H2O2 Standard (0.88 M)||Yellow||1 x 0.1ml|
|OxiRed™ Probe (in DMSO)||Red||1 x 200µl|
|XO Assay Buffer||WM||1 x 25ml|
|XO Enzyme Mix (Lyophilized)||Green||1 vial|
|XO Positive Control||Blue||1 x 8µl|
|XO Substrate Mix (Lyophilized)||Purple||1 vial|
Our Abpromise guarantee covers the use of ab102522 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|