Overview

  • Product nameAnti-Xanthine Oxidase antibody
    See all Xanthine Oxidase primary antibodies
  • Description
    Rabbit polyclonal to Xanthine Oxidase
  • SpecificityCross-reactivity against Xanthine Oxidase from other tissues and species may occur but have not been specifically determined.
  • Tested applicationsSuitable for: WB, IP, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Cow, Human
  • Immunogen

    Native protein (purified) Bovine buttermilk Xanthine Oxidase

  • Positive control
    • Liver tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab85541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 146 kDa.
IP Use at an assay dependent concentration.
ELISA 1/100000 - 1/400000.
IHC-P Use a concentration of 2.5 µg/ml.

Target

  • FunctionKey enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro).
  • Tissue specificityDetected in milk (at protein level).
  • Involvement in diseaseDefects in XDH are the cause of xanthinuria type 1 (XU1) [MIM:278300]. Xanthinuria is characterized by excretion of very large amounts of xanthine in the urine and a tendency to form xanthine stones. Uric acid is strikingly diminished in serum and urine. XU1 is due to isolated xanthine dehydrogenase. XU1 patients can metabolize allopurinol.
  • Sequence similaritiesBelongs to the xanthine dehydrogenase family.
    Contains 1 2Fe-2S ferredoxin-type domain.
    Contains 1 FAD-binding PCMH-type domain.
  • Post-translational
    modifications
    Subject to partial proteolysis; this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).
    Contains sulfhydryl groups that are easily oxidized (in vitro); this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).
  • Cellular localizationCytoplasm. Peroxisome. Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Xanthine dehydrogenase antibody
    • Xanthine dehydrogenase/oxidase antibody
    • Xanthine oxidase antibody
    • Xanthine oxidoreductase antibody
    • XD antibody
    • XDH antibody
    • XDH_HUMAN antibody
    • xdha antibody
    • XO antibody
    • xor antibody
    see all

Anti-Xanthine Oxidase antibody images

  • ab85541 at 2.5µg/ml staining Xanthine Oxidase in Formalin-Fixed Paraffin-Embedded human liver tissue.
    A after heat induced antigen retrieval step was performed using pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

References for Anti-Xanthine Oxidase antibody (ab85541)

ab85541 has not yet been referenced specifically in any publications.

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