Anti-Xanthine Oxidase antibody [EPR4605] (ab109235)

Overview

  • Product nameAnti-Xanthine Oxidase antibody [EPR4605]
    See all Xanthine Oxidase primary antibodies
  • Description
    Rabbit monoclonal [EPR4605] to Xanthine Oxidase
  • Tested applicationsSuitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (internal sequence)

  • Positive control
    • Human fetal liver, 293T, Human milk, and Mouse liver lysates; Human liver tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab109235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 146 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt,ICC or IP.
  • Target

    • FunctionKey enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro).
    • Tissue specificityDetected in milk (at protein level).
    • Involvement in diseaseDefects in XDH are the cause of xanthinuria type 1 (XU1) [MIM:278300]. Xanthinuria is characterized by excretion of very large amounts of xanthine in the urine and a tendency to form xanthine stones. Uric acid is strikingly diminished in serum and urine. XU1 is due to isolated xanthine dehydrogenase. XU1 patients can metabolize allopurinol.
    • Sequence similaritiesBelongs to the xanthine dehydrogenase family.
      Contains 1 2Fe-2S ferredoxin-type domain.
      Contains 1 FAD-binding PCMH-type domain.
    • Post-translational
      modifications
      Subject to partial proteolysis; this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).
      Contains sulfhydryl groups that are easily oxidized (in vitro); this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).
    • Cellular localizationCytoplasm. Peroxisome. Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Xanthine dehydrogenase antibody
      • Xanthine dehydrogenase/oxidase antibody
      • Xanthine oxidase antibody
      • Xanthine oxidoreductase antibody
      • XD antibody
      • XDH antibody
      • XDH_HUMAN antibody
      • xdha antibody
      • XO antibody
      • xor antibody
      see all

    Anti-Xanthine Oxidase antibody [EPR4605] images

    • All lanes : Anti-Xanthine Oxidase antibody [EPR4605] (ab109235) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : 293T lysate
      Lane 3 : Human milk lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 146 kDa

      Secondary antibody - anti-rabbit HRP (ab6721)

    • Anti-Xanthine Oxidase antibody [EPR4605] (ab109235) at 1/1000 dilution + Mouse liver lysate at 10 µg

      Predicted band size : 146 kDa

      Secondary antibody - anti-rabbit HRP (ab6721)

    • Immunohistochemical analysis of Xanthine Oxidase expression in paraffin-embedded Human liver tissue using ab109235 at 1/100 dilution.

    References for Anti-Xanthine Oxidase antibody [EPR4605] (ab109235)

    This product has been referenced in:
    • Brem R & Karran P Oxidation-mediated DNA cross-linking contributes to the toxicity of 6-thioguanine in human cells. Cancer Res 72:4787-95 (2012). WB ; Human . Read more (PubMed: 22822082) »

    See 1 Publication for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Equus Tissue sections (Jejunum)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate, pH6 (heat-induced epitope retrieval)
    Permeabilization No
    Specification Jejunum
    Blocking step Background Buster (Innovex) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 23°C
    Fixative Formaldehyde
    Username

    Esther Gu

    Verified customer

    Submitted Jul 24 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Liver, kidney)
    Loading amount 40 µg
    Specification Liver, kidney
    Gel Running Conditions Reduced Denaturing (8%)
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 17 2012

    Thank you very much for your reply and for the information.

    We would be very pleased indeed to update the product datasheet with your results, I therefore would like to suggest you to submit an Abreview via the online product datasheet. We alw...

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    Thank you for your reply and this further information.
    My colleagues from the lab and I would be tempted to just say "yes, the antibody recognises the xanthine reductase at 145kDa as well as the xanthine oxidase at around 80kDa". Our only...

    Read More

    Thank you for contacting us.

    My colleagues from the lab confirmed that, based on our in house data, and the information provided by Uniprot, the predicted MW of XDH is 150kDa.
    Do you have any knowledge or information on different subunits...

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    DISCOUNT CODES:
    Ab109235: ***
    Ab32675: ***

    Expiration date: October 5, 2012

    Thesecodes will each give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for each antibody...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"