Overview

  • Product name
  • Description
    Rabbit polyclonal to XBP1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human XBP1.

    (Peptide available as ab30769.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: Jurkat Whole Cell, A431 Whole Cell, Rat Testis Tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab28715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 33 kDa). We believe this band represents the protein product of the unprocessed XBP1 mRNA that is present in the absence of a stress response.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Transcription factor essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response (UPR). Acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I.
    • Involvement in disease
      Genetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7) [MIM:612371]. Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature.
    • Sequence similarities
      Belongs to the bZIP family.
      Contains 1 bZIP domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Tax responsive element binding protein 5 antibody
      • Tax-responsive element-binding protein 5 antibody
      • TREB5 antibody
      • X box binding protein 1 antibody
      • X box binding protein 2 antibody
      • X-box-binding protein 1 antibody
      • XBP 1 antibody
      • XBP-1 antibody
      • XBP1 antibody
      • XBP1_HUMAN antibody
      • XBP2 antibody
      see all

    Images

    • All lanes : Anti-XBP1 antibody (ab28715) at 1 µg/ml

      Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Testis (Rat) Tissue Lysate
      Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 33 kDa
      Observed band size : 35 kDa (why is the actual band size different from the predicted?)


      Exposure time : 8 minutes

    References

    ab28715 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab28715.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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