Synthetic peptide corresponding to 18 amino acids near to the N terminus of the human XBP1 protein.
Our Abpromise guarantee covers the use of ab37152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 20028872Use at an assay dependent dilution (PMID 20028872).|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 29 kDa.Can be blocked with Human XBP1 peptide (ab39910). Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 29 kDa(isoform 1) and 40 kD (isoform 2). Can be blocked with XBP1 peptide (ab39910).|
|ICC/IF||Use a concentration of 10 µg/ml.|
|ELISA||Use at an assay dependent concentration.|
ab37152 at 1/200 dilution staining XBP1 in human non-lesional skin tissue sections by Immunohistochemistry (Formalin/ PFA fixed paraformaldehyde tissue sections). A biotin labelled secondary was used at 1/100 dilution. Diaminobenzidine (DAB) was used for staining development in a ABC staining Kit and the sections were counterstained with haematoxylin.