Anti-XBP1 antibody (ab85546)

Overview

  • Product nameAnti-XBP1 antibody
    See all XBP1 primary antibodies
  • Description
    Goat polyclonal to XBP1
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Dog, Human
    Predicted to work with: Mouse, Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide:

    NHSWEDTFANELFPQ

    by a Cysteine residue linker, corresponding to C terminal amino acids 358-372 of Human XBP1 isoform 2

  • Positive control
    • Human breast tissue; HeLa nuclear lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab85546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 40 kDa.
ELISA 1/4000.
IHC-P Use a concentration of 5 µg/ml.

Target

  • FunctionTranscription factor essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response (UPR). Acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I.
  • Involvement in diseaseGenetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7) [MIM:612371]. Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature.
  • Sequence similaritiesBelongs to the bZIP family.
    Contains 1 bZIP domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Tax responsive element binding protein 5 antibody
    • Tax-responsive element-binding protein 5 antibody
    • TREB5 antibody
    • X box binding protein 1 antibody
    • X box binding protein 2 antibody
    • X-box-binding protein 1 antibody
    • XBP 1 antibody
    • XBP-1 antibody
    • XBP1 antibody
    • XBP1_HUMAN antibody
    • XBP2 antibody
    see all

Anti-XBP1 antibody images

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded human breast tissue using ab85546 at a dilution of 5µg/ml.
  • Anti-XBP1 antibody (ab85546) at 1 µg/ml + nuclear HeLa lysate in RIPA buffer at 35 µg

    Predicted band size : 40 kDa

References for Anti-XBP1 antibody (ab85546)

ab85546 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Liver)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrat-buffer
Permeabilization No
Specification Liver
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 22°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 11 2015

Thank you very much for your call yesterday and for forwarding this dog sequence to me.

Looking at the alignment results, I do think it is quite possible for ab37152 to cross-react with your dog samples. I'm sending your information to Cedarl...

Read More

Thank you for your call today and for your interest in our XBP1 antibodies. Please see below for information about your testing code, and let me know if you have any questions or if there is anything else that we can do for you.

DISCOUNT CODE...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"