Recombinant
RabMAb

Anti-XBP1 antibody [EPR4086] (ab109221)

Abreviews (1) Submit a Question Specific References (4)

Overview

  • Product name
    Anti-XBP1 antibody [EPR4086]
    See all XBP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4086] to XBP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human XBP1 aa 100-200.
    Database link: P17861

  • Positive control
    • Fetal heart, fetal kidney, HT-29, 293T, and Jurkat cell lysates Human colon tissue Human urinary bladder transitional carcinoma tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab109221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
IHC-P 1/500 - 1/1000. Perform antigen retrieval
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function
      Transcription factor essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response (UPR). Acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I.
    • Involvement in disease
      Genetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7) [MIM:612371]. Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature.
    • Sequence similarities
      Belongs to the bZIP family.
      Contains 1 bZIP domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
      • Alternative names
        • Tax responsive element binding protein 5 antibody
        • Tax-responsive element-binding protein 5 antibody
        • TREB5 antibody
        • X box binding protein 1 antibody
        • X box binding protein 2 antibody
        • X-box-binding protein 1 antibody
        • XBP 1 antibody
        • XBP-1 antibody
        • XBP1 antibody
        • XBP1_HUMAN antibody
        • XBP2 antibody
        see all

      Images

      • Flow Cytometry - Anti-XBP1 antibody [EPR4086] (ab109221)
        Flow Cytometry - Anti-XBP1 antibody [EPR4086] (ab109221)
        Overlay histogram showing HepG2 cells stained with ab109221 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109221, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
      • Western blot - XBP1 antibody [EPR4086] (ab109221)
        Western blot - XBP1 antibody [EPR4086] (ab109221)
        All lanes : Anti-XBP1 antibody [EPR4086] (ab109221) at 1/1000 dilution

        Lane 1 : Fetal heart cell lysates
        Lane 2 : Fetal kidney cell Fysates
        Lane 3 : HT-29 cell lysates
        Lane 4 : 293T cell lysates
        Lane 5 : Jurkat cell lysates

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 29 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XBP1 antibody [EPR4086] (ab109221)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XBP1 antibody [EPR4086] (ab109221)
        Immunohistochemical analysis of paraffin-embedded Human colon tissue using ab109221 at 1/500.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XBP1 antibody [EPR4086] (ab109221)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XBP1 antibody [EPR4086] (ab109221)
        Immunohistochemical analysis of paraffin-embedded Human urinary bladder transitional carcinoma tissue using ab109221 at 1/500.

      References

      This product has been referenced in:
      • Meng T  et al. Tat-haFGF14-154 Upregulates ADAM10 to Attenuate the Alzheimer Phenotype of APP/PS1 Mice through the PI3K-CREB-IRE1a/XBP1 Pathway. Mol Ther Nucleic Acids 7:439-452 (2017). WB ; Mouse . Read more (PubMed: 28624220) »
      • Wang WF  et al. HSP70-Hrd1 axis precludes the oncorepressor potential of N-terminal misfolded Blimp-1s in lymphoma cells. Nat Commun 8:363 (2017). Read more (PubMed: 28842558) »

      See all 4 Publications for this product

      Publishing research using ab109221? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      1 Review or Q&A

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      Western blot abreview for Anti-XBP1 antibody [EPR4086]

       Excellent
      Abreviews
      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Lymphocytes)
      Gel Running Conditions
      Reduced Denaturing (4-12% bis-tris)
      Loading amount
      20 µg
      Specification
      Lymphocytes
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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      Submitted Sep 22 2017

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