Recombinant
Anti-XBP1 antibody [EPR4086] (ab109221)
- Datasheet
- Specific References (4)
- Protocols
Overview
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Product nameAnti-XBP1 antibody [EPR4086]
See all XBP1 primary antibodies -
DescriptionRabbit monoclonal [EPR4086] to XBP1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Unsuitable for: IP -
Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human XBP1 aa 100-200. The exact sequence is proprietary.
Database link: P17861 -
Positive control
- Fetal heart, fetal kidney, HT-29, 293T, and Jurkat cell lysates Human colon tissue Human urinary bladder transitional carcinoma tissue
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsFrozen Stock (-20C). Shelf life 12 months.
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Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR4086
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab109221 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 29 kDa. | |
| IHC-P | 1/500 - 1/1000. Perform antigen retrieval | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | 1/250 - 1/500. |
Application notes
Is unsuitable for IP.
Target
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FunctionTranscription factor essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response (UPR). Acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I.
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Involvement in diseaseGenetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7) [MIM:612371]. Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature.
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Sequence similaritiesBelongs to the bZIP family.
Contains 1 bZIP domain. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 7494 Human
- Omim: 194355 Human
- SwissProt: P17861 Human
- Unigene: 437638 Human
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Alternative names
- Tax responsive element binding protein 5 antibody
- Tax-responsive element-binding protein 5 antibody
- TREB5 antibody
see all
Images
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![Flow Cytometry - Anti-XBP1 antibody [EPR4086] (ab109221)](http://a.abcam.com/ps/products/109/ab109221/Images/ab109221-4-ab109221FC.jpg)
Flow Cytometry - Anti-XBP1 antibody [EPR4086] (ab109221)Overlay histogram showing HepG2 cells stained with ab109221 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109221, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. -
![Western blot - Anti-XBP1 antibody [EPR4086] (ab109221)](http://a.abcam.com/ps/products/109/ab109221/Images/XBP1-Primary-antibodies-ab109221-3.jpg)
Western blot - Anti-XBP1 antibody [EPR4086] (ab109221)All lanes : Anti-XBP1 antibody [EPR4086] (ab109221) at 1/1000 dilution
Lane 1 : Fetal heart cell lysates
Lane 2 : Fetal kidney cell Fysates
Lane 3 : HT-29 cell lysates
Lane 4 : 293T cell lysates
Lane 5 : Jurkat cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 29 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody [EPR4086] (ab109221)](http://a.abcam.com/ps/products/109/ab109221/Images/XBP1-Primary-antibodies-ab109221-5.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody [EPR4086] (ab109221)Immunohistochemical analysis of paraffin-embedded Human colon tissue using ab109221 at 1/500. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody [EPR4086] (ab109221)](http://a.abcam.com/ps/products/109/ab109221/Images/XBP1-Primary-antibodies-ab109221-6.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody [EPR4086] (ab109221)Immunohistochemical analysis of paraffin-embedded Human urinary bladder transitional carcinoma tissue using ab109221 at 1/500.
Protocols
References
This product has been referenced in:
- Meng T et al. Tat-haFGF14-154 Upregulates ADAM10 to Attenuate the Alzheimer Phenotype of APP/PS1 Mice through the PI3K-CREB-IRE1a/XBP1 Pathway. Mol Ther Nucleic Acids 7:439-452 (2017). WB ; Mouse . Read more (PubMed: 28624220) »
- Wang WF et al. HSP70-Hrd1 axis precludes the oncorepressor potential of N-terminal misfolded Blimp-1s in lymphoma cells. Nat Commun 8:363 (2017). Read more (PubMed: 28842558) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"