Overview

  • Product name
  • Description
    Goat polyclonal to xCT
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Pig, Orangutan
  • Immunogen

    Synthetic peptide:

    KGQTQNFKDAFSGRD

    by a Cysteine residue linker, corresponding to internal sequence amino acids 215-229 of Human xCT.

  • Positive control
    • Human Kidney and Placenta tissues; Human Spleen lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab111822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/128000.
WB Use a concentration of 0.02 - 0.06 µg/ml. Predicted molecular weight: 55 kDa.
IHC-P Use a concentration of 3.75 µg/ml.

Target

  • Function
    Sodium-independent, high-affinity exchange of anionic amino acids with high specificity for anionic form of cystine and glutamate.
  • Sequence similarities
    Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Amino acid transport system xc xCT antibody antibody
    • Amino acid transport system xc- antibody
    • Calcium channel blocker resistance protein CCBR1 antibody
    • Calcium channel blocker resistance protein CCBR1 antibody antibody
    • CCBR1 antibody
    • Cysteine/glutamate transporter antibody antibody
    • Cystine/glutamate transporter antibody
    • OTTHUMP00000164578 antibody
    • SLC7A11 antibody
    • Solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11 antibody
    • solute carrier family 7 antibody
    • Solute carrier family 7 member 11 antibody
    • Solute carrier family 7, (cationic amino acid transporter, y+ system) member 11 antibody
    • SYSTEM Xc(-) TRANSPORTER-RELATED PROTEIN antibody
    • xCT antibody
    • XCT_HUMAN antibody
    see all

Images

  • ab111822 at 3.75 µg/ml staining xCT in Formalin-Fixed, Paraffin-Embedded Human Kidney tissue by Immunohistochemistry.
  • ab111822 at 3.75 µg/ml staining xCT in Formalin-Fixed, Paraffin-Embedded Human Placenta tissue by Immunohistochemistry.
  • Anti-xCT antibody (ab111822) at 0.02 µg/ml (for one hour) + Human Spleen lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 55 kDa

References

This product has been referenced in:
  • Ju HQ  et al. Redox Regulation of Stem-like Cells Though the CD44v-xCT Axis in Colorectal Cancer: Mechanisms and Therapeutic Implications. Theranostics 6:1160-75 (2016). Read more (PubMed: 27279909) »

See 1 Publication for this product

Customer reviews and Q&As

We have added product ab178004, xCT peptide, as a blocking peptide to be used with ab111822. Here is a link: http://www.abcam.com/xct-peptide-ab178004.html.

Application
Flow Cytometry
Sample
Human Cell (liver hepatocellular cells HepG2)
Specification
liver hepatocellular cells HepG2
Preparation
Cell harvesting/tissue preparation method: trypsinized and resuspended in PBS+1% serum+0.1% NaN3
Sample buffer: PBS+1% serum+0.1% NaN3
Fixation
did not fix
Permeabilization
No
Gating Strategy
ungated cell population
Username

Yuzhe

Verified customer

Submitted Sep 05 2012

Thank you for contacting us. You are correct, ab111822 is raised against an extracellular region of xCT. As such you should not have to permeablize your cells to use this product. I hope that this information is helpful. Please let me know if y...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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