Overview

  • Product nameAnti-xCT antibodySee all xCT primary antibodies ...
  • Description
    Rabbit polyclonal to xCT
  • SpecificityThis antibody is specific for XCT.
  • Tested applicationsWB, Flow Cyt, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide: MVRKPVVSTI SKGGYLQGNV NGRLPSLGNK EPPGQEKVQL , corresponding to N terminal amino acids 1/50 of Human xCT

  • Positive control
    • human and mouse Stomach tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab37185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 55 kDa. (ECL).
Flow Cyt Use at an assay dependent dilution.
IHC-FoFr Use at an assay dependent dilution. PubMed: 21639880

Target

  • FunctionSodium-independent, high-affinity exchange of anionic amino acids with high specificity for anionic form of cystine and glutamate.
  • Sequence similaritiesBelongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Amino acid transport system xc xCT antibody antibody
    • Amino acid transport system xc- antibody
    • Calcium channel blocker resistance protein CCBR1 antibody
    • Calcium channel blocker resistance protein CCBR1 antibody antibody
    • CCBR1 antibody
    • Cysteine/glutamate transporter antibody antibody
    • cystine/glutamate transporter antibody
    • OTTHUMP00000164578 antibody
    • SLC7A11 antibody
    • Solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11 antibody
    • solute carrier family 7 antibody
    • Solute carrier family 7 member 11 antibody
    • Solute carrier family 7, (cationic amino acid transporter, y+ system) member 11 antibody
    • SYSTEM Xc(-) TRANSPORTER-RELATED PROTEIN antibody
    • xCT antibody
    • XCT_HUMAN antibody
    see all

Anti-xCT antibody images

  • All lanes : Anti-xCT antibody (ab37185) at 0.5 µg/ml

    Lane 1 : Total human stomach lysate.
    Lane 2 : Total mouse stomach lysate.

    developed using the ECL technique

    Predicted band size : 55 kDa
    Observed band size : 55 kDa


    Exposure time : 1 minute

References for Anti-xCT antibody (ab37185)

This product has been referenced in:
  • Mitchell LA  et al. Suppression of vaccine immunity by inflammatory monocytes. J Immunol 189:5612-21 (2012). Flow Cyt ; Mouse . Read more (PubMed: 23136203) »
  • Pampliega O  et al. Increased expression of cystine/glutamate antiporter in multiple sclerosis. J Neuroinflammation 8:63 (2011). WB, Flow Cyt, IHC-FoFr ; Human, Mouse, Rat . Read more (PubMed: 21639880) »

See all 5 Publications for this product

Product Wall

Thanks so muchfor your reply and best of luck with your upcoming experiments. If you have any further questions please do not hesitate to contact me.

Thanks for submitting your enquiry. I am sorry to hear this antibody is giving you trouble.

I was just wondering what positive control samples you have used?

Thanks in advance for the additional information.

Thank you for contacting us and making us aware of this. We have been transitioning to a new email system and it may be that the response which I had sent on Mon 16 Apr 12 13:26GMT was somehow caught up or perhaps it went directly to your spam or ju...

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Thank you for your response and for the additional details which you have provided.

You are correct as this antibody will only recognize the intracellular epitope of xCT. As such you would need to permeablize your cells to allow entry of the...

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Thank you for contacting Abcam, I am sorry to hear that you have been experiencing difficulties using this product in flow.

Would you be able to provide me the specific details of your experiments? Useful information would include but not be...

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Thank you for contacting Abcam.

We are unable to send you any free/trail sizes of our antibodies. When your ordering system is reorganized at the end of the month, you will be able to order the antibody and if it is in stock then it will be s...

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Thank you for your call today and for letting us know about the trouble with ab37185. As we discussed, please let me know if the suggestions that we discussed don't improve the results, and I will be happy to help you further. If you have any questions...

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Thank you for your enquiry. As discussed on the phone, here is the email that was sent in response to your questionnaire from Kate Hayes on December 1st, 2006. Reply from Abcam to your enquiry regarding ab37185 Dear Ilangovan, In response to ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"