Overview

  • Product name
  • Description
    Rabbit polyclonal to xCT
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-P, ICC/IF, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human xCT aa 1-50 (N terminal). The exact sequence is proprietary.
    Sequence:

    MVRKPVVSTISKGGYLQGNVNGRLPSLGNKEPPGQEKVQLKRKVTLLRGV


    Database link: Q9UPY5

  • Positive control
    • WB: Human and mouse stomach tissue lysate; Human Stomach Cancer tissue lysate; HepG2 cell lysate IHC: Small intestine ICC/IF: HepG2 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab37185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 55 kDa.

xCT forms heterodimer with 4F2Hc protein hence reducing conditions should be used in WB. It exists as multi-pass membrane protein therefore we recommend heating the lysates for 15-20 minutes at 60-70C.

Flow Cyt Use a concentration of 2 - 3 µg/ml.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use a concentration of 5 µg/ml.
ICC/IF 1/100 - 1/1000.
IHC-FoFr Use at an assay dependent concentration. PubMed: 21639880

Target

  • Function
    Sodium-independent, high-affinity exchange of anionic amino acids with high specificity for anionic form of cystine and glutamate.
  • Sequence similarities
    Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Amino acid transport system xc xCT antibody antibody
    • Amino acid transport system xc- antibody
    • Calcium channel blocker resistance protein CCBR1 antibody
    • Calcium channel blocker resistance protein CCBR1 antibody antibody
    • CCBR1 antibody
    • Cysteine/glutamate transporter antibody antibody
    • Cystine/glutamate transporter antibody
    • OTTHUMP00000164578 antibody
    • SLC7A11 antibody
    • Solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11 antibody
    • solute carrier family 7 antibody
    • Solute carrier family 7 member 11 antibody
    • Solute carrier family 7, (cationic amino acid transporter, y+ system) member 11 antibody
    • SYSTEM Xc(-) TRANSPORTER-RELATED PROTEIN antibody
    • xCT antibody
    • XCT_HUMAN antibody
    see all

Anti-xCT antibody images

  • All lanes : Anti-xCT antibody (ab37185) at 0.5 µg/ml

    Lane 1 : Total human stomach lysate.
    Lane 2 : Total mouse stomach lysate.

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique

    Predicted band size : 55 kDa
    Observed band size : 55 kDa


    Exposure time : 1 minute
  • Imunocytochemistry/ Immunofluorescence analysis of HepG2 cells labeling xCT with ab37185 followed by Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red) respectively. 

  • Immunohistochemical analysis of small intestine labeling xCT with ab37185. Staining is observed in the absorptive epithelia of small intestinal villi. 

  • PFA perfusion fixed frozen sections of spinal cord of control (left) and MS patients (right) stained for xCT using ab37185 at 5 µg/ml.

  • All lanes : Anti-xCT antibody (ab37185)

    Lane 1 : Human Stomach Cancer tissue lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 0.5 mg/ml per lane.


    Predicted band size : 55 kDa

    This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa.

References for Anti-xCT antibody (ab37185)

This product has been referenced in:
  • Zhang P  et al. xCT expression modulates cisplatin resistance in Tca8113 tongue carcinoma cells. Oncol Lett 12:307-314 (2016). Read more (PubMed: 27347143) »
  • Cormerais Y  et al. Genetic Disruption of the Multifunctional CD98/LAT1 Complex Demonstrates the Key Role of Essential Amino Acid Transport in the Control of mTORC1 and Tumor Growth. Cancer Res 76:4481-92 (2016). WB ; Human . Read more (PubMed: 27302165) »

See all 12 Publications for this product

Product Wall

Application
Western blot
Sample
Human Cell lysate - whole cell (head and neck cancer)
Gel Running Conditions
Non-reduced Denaturing (10% gel)
Loading amount
20 µg
Treatment
drug 48h
Specification
head and neck cancer
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 21 2015

Application
IHC - Wholemount
Sample
Human Tissue (head and neck cancer)
Specification
head and neck cancer
Username

Miss. Jin Young Park

Verified customer

Submitted Oct 19 2015

Thanks so muchfor your reply and best of luck with your upcoming experiments. If you have any further questions please do not hesitate to contact me.

Thanks for submitting your enquiry. I am sorry to hear this antibody is giving you trouble.

I was just wondering what positive control samples you have used?

Thanks in advance for the additional information.

Thank you for contacting us and making us aware of this. We have been transitioning to a new email system and it may be that the response which I had sent on Mon 16 Apr 12 13:26GMT was somehow caught up or perhaps it went directly to your spam or ju...

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Thank you for your response and for the additional details which you have provided.

You are correct as this antibody will only recognize the intracellular epitope of xCT. As such you would need to permeablize your cells to allow entry of the...

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Thank you for contacting Abcam, I am sorry to hear that you have been experiencing difficulties using this product in flow.

Would you be able to provide me the specific details of your experiments? Useful information would include but not be...

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Thank you for contacting Abcam.

We are unable to send you any free/trail sizes of our antibodies. When your ordering system is reorganized at the end of the month, you will be able to order the antibody and if it is in stock then it will be s...

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Thank you for your call today and for letting us know about the trouble with ab37185. As we discussed, please let me know if the suggestions that we discussed don't improve the results, and I will be happy to help you further. If you have any questions...

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Thank you for your enquiry. As discussed on the phone, here is the email that was sent in response to your questionnaire from Kate Hayes on December 1st, 2006. Reply from Abcam to your enquiry regarding ab37185 Dear Ilangovan, In response to ...

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