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  • Product nameAnti-xCT antibody
    See all xCT primary antibodies
  • Description
    Rabbit polyclonal to xCT
  • SpecificityThis antibody is specific for XCT.
  • Tested applicationsWB, Flow Cyt, IHC-P, ICC/IF, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide: MVRKPVVSTI SKGGYLQGNV NGRLPSLGNK EPPGQEKVQL , corresponding to N terminal amino acids 1/50 of Human xCT

  • Positive control
    • human and mouse Stomach tissue lysate.



Our Abpromise guarantee covers the use of ab37185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 55 kDa.

xCT forms heterodimer with 4F2Hc protein hence reducing conditions should be used in WB. It exists as multi-pass membrane protein therefore we recommend heating the lysates for 15-20 minutes at 60-70C.

Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 100 - 1000 µg/ml.
IHC-FoFr Use at an assay dependent concentration. PubMed: 21639880


  • FunctionSodium-independent, high-affinity exchange of anionic amino acids with high specificity for anionic form of cystine and glutamate.
  • Sequence similaritiesBelongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Amino acid transport system xc xCT antibody antibody
    • Amino acid transport system xc- antibody
    • Calcium channel blocker resistance protein CCBR1 antibody
    • Calcium channel blocker resistance protein CCBR1 antibody antibody
    • CCBR1 antibody
    • Cysteine/glutamate transporter antibody antibody
    • Cystine/glutamate transporter antibody
    • OTTHUMP00000164578 antibody
    • SLC7A11 antibody
    • Solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11 antibody
    • solute carrier family 7 antibody
    • Solute carrier family 7 member 11 antibody
    • Solute carrier family 7, (cationic amino acid transporter, y+ system) member 11 antibody
    • xCT antibody
    • XCT_HUMAN antibody
    see all

Anti-xCT antibody images

  • All lanes : Anti-xCT antibody (ab37185) at 0.5 µg/ml

    Lane 1 : Total human stomach lysate.
    Lane 2 : Total mouse stomach lysate.

    developed using the ECL technique

    Predicted band size : 55 kDa
    Observed band size : 55 kDa

    Exposure time : 1 minute

References for Anti-xCT antibody (ab37185)

This product has been referenced in:
  • Ye P  et al. Nrf2- and ATF4-dependent upregulation of xCT modulates the sensitivity of T24 bladder carcinoma cells to proteasome inhibition. Mol Cell Biol 34:3421-34 (2014). Read more (PubMed: 25002527) »
  • Mitchell LA  et al. Suppression of vaccine immunity by inflammatory monocytes. J Immunol 189:5612-21 (2012). Flow Cyt ; Mouse . Read more (PubMed: 23136203) »

See all 6 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (head and neck cancer)
Gel Running Conditions Non-reduced Denaturing (10% gel)
Loading amount 20 µg
Treatment drug 48h
Specification head and neck cancer
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 21 2015

Application IHC - Wholemount
Sample Human Tissue (head and neck cancer)
Specification head and neck cancer

Miss. jin young park

Verified customer

Submitted Oct 19 2015

Thanks so muchfor your reply and best of luck with your upcoming experiments. If you have any further questions please do not hesitate to contact me.

Thanks for submitting your enquiry. I am sorry to hear this antibody is giving you trouble.

I was just wondering what positive control samples you have used?

Thanks in advance for the additional information.

Thank you for contacting us and making us aware of this. We have been transitioning to a new email system and it may be that the response which I had sent on Mon 16 Apr 12 13:26GMT was somehow caught up or perhaps it went directly to your spam or ju...

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Thank you for your response and for the additional details which you have provided.

You are correct as this antibody will only recognize the intracellular epitope of xCT. As such you would need to permeablize your cells to allow entry of the...

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Thank you for contacting Abcam, I am sorry to hear that you have been experiencing difficulties using this product in flow.

Would you be able to provide me the specific details of your experiments? Useful information would include but not be...

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Thank you for contacting Abcam.

We are unable to send you any free/trail sizes of our antibodies. When your ordering system is reorganized at the end of the month, you will be able to order the antibody and if it is in stock then it will be s...

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Thank you for your call today and for letting us know about the trouble with ab37185. As we discussed, please let me know if the suggestions that we discussed don't improve the results, and I will be happy to help you further. If you have any questions...

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Thank you for your enquiry. As discussed on the phone, here is the email that was sent in response to your questionnaire from Kate Hayes on December 1st, 2006. Reply from Abcam to your enquiry regarding ab37185 Dear Ilangovan, In response to ...

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