Customer reviews and FAQs

Overview

  • Product nameAnti-xCT antibody
    See all xCT primary antibodies
  • Description
    Rabbit polyclonal to xCT
  • SpecificityThis antibody is specific for XCT.
  • Tested applicationsSuitable for: WB, Flow Cyt, IHC-P, ICC/IF, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide: MVRKPVVSTI SKGGYLQGNV NGRLPSLGNK EPPGQEKVQL , corresponding to N terminal amino acids 1/50 of Human xCT

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • human and mouse Stomach tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab37185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 55 kDa.

xCT forms heterodimer with 4F2Hc protein hence reducing conditions should be used in WB. It exists as multi-pass membrane protein therefore we recommend heating the lysates for 15-20 minutes at 60-70C.
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 100 - 1000 µg/ml.
IHC-FoFr Use at an assay dependent concentration. PubMed: 21639880

Target

  • FunctionSodium-independent, high-affinity exchange of anionic amino acids with high specificity for anionic form of cystine and glutamate.
  • Sequence similaritiesBelongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • Amino acid transport system xc xCT antibody antibody
      • Amino acid transport system xc- antibody
      • Calcium channel blocker resistance protein CCBR1 antibody
      • Calcium channel blocker resistance protein CCBR1 antibody antibody
      • CCBR1 antibody
      • Cysteine/glutamate transporter antibody antibody
      • Cystine/glutamate transporter antibody
      • OTTHUMP00000164578 antibody
      • SLC7A11 antibody
      • Solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11 antibody
      • solute carrier family 7 antibody
      • Solute carrier family 7 member 11 antibody
      • Solute carrier family 7, (cationic amino acid transporter, y+ system) member 11 antibody
      • SYSTEM Xc(-) TRANSPORTER-RELATED PROTEIN antibody
      • xCT antibody
      • XCT_HUMAN antibody
      see all

    Anti-xCT antibody images

    • Western blot - xCT antibody (ab37185)
      Western blot - xCT antibody (ab37185)
      All lanes : Anti-xCT antibody (ab37185) at 0.5 µg/ml

      Lane 1 : Total human stomach lysate.
      Lane 2 : Total mouse stomach lysate.

      developed using the ECL technique

      Predicted band size : 55 kDa
      Observed band size : 55 kDa


      Exposure time : 1 minute

    References for Anti-xCT antibody (ab37185)

    This product has been referenced in:
    • Evonuk KS  et al. Inhibition of System Xc(-) Transporter Attenuates Autoimmune Inflammatory Demyelination. J Immunol 195:450-63 (2015). Read more (PubMed: 26071560) »
    • Ye P  et al. Nrf2- and ATF4-dependent upregulation of xCT modulates the sensitivity of T24 bladder carcinoma cells to proteasome inhibition. Mol Cell Biol 34:3421-34 (2014). Read more (PubMed: 25002527) »

    See all 7 Publications for this product

    Publishing research using ab37185? Please let us know so that we can cite the reference in this datasheet.

    Product Wall

    Submit an abreview for ab37185 Submit a question for ab37185

    10 Abreviews or Q&A

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    Votes: Highest First

    Western blot abreview for Anti-xCT antibody

     Average
    Abreviews
    Application Western blot
    Sample Human Cell lysate - whole cell (head and neck cancer)
    Gel Running Conditions Non-reduced Denaturing (10% gel)
    Loading amount 20 µg
    Treatment drug 48h
    Specification head and neck cancer
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted Oct 21 2015

    IHC - Wholemount abreview for Anti-xCT antibody

     Excellent
    Abreviews
    Application IHC - Wholemount
    Sample Human Tissue (head and neck cancer)
    Specification head and neck cancer
    Read More
    abreview image
    Username

    Miss. jin young park

    Verified customer

    Submitted Oct 19 2015



    You ask a good question. Actually, A549 cells have been shown to have high expression of xCT, so that is my positive control. After doing a bit of research on the matter, I think that the 105 kDa band that I am detecting is probably the hete...

    Read More

    Thanks so muchfor your reply and best of luck with your upcoming experiments. If you have any further questions please do not hesitate to contact me.

    Inquiry: Hello, I have made two attempts to use your anti-xCT antibody to detect xCT in cell lysates and both blots have resulted in numerous bands, none at the predicted MW of 55 kDa. I have attached an image of my most recent blot to this message. My...

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    Thanks for submitting your enquiry. I am sorry to hear this antibody is giving you trouble.

    I was just wondering what positive control samples you have used?

    Thanks in advance for the additional information.

    Hi There,

     

    I wrote you emails to explain my concern of using your xCT antibody to do flow cytometry and ask for your help. But I did not receive any response after I provided you with my experiment details, which makes me very up...

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    Thank you for contacting us and making us aware of this. We have been transitioning to a new email system and it may be that the response which I had sent on Mon 16 Apr 12 13:26GMT was somehow caught up or perhaps it went directly to your spam or ju...

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    I used human breast cancer cell line MCF-7 cells. Negative control: cells only, secondary anti rabbit IgG fitc only. No positive control. I used up the antibody for real experiments.. FITC secondary antibody was newly ordered, it is working very wel...

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    Thank you for your response and for the additional details which you have provided.

    You are correct as this antibody will only recognize the intracellular epitope of xCT. As such you would need to permeablize your cells to allow entry of the...

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    Hi There,

    Our lab recently purchased xCT antibody ab37185 for flow cytometry study. I want to measure xCT cell surface expression. So I first incubated my cells with ab37185 (10ul per sample) for 1hr and washed for 2˜3 times with PBS then incu...

    Read More

    Thank you for contacting Abcam, I am sorry to hear that you have been experiencing difficulties using this product in flow.

    Would you be able to provide me the specific details of your experiments? Useful information would include but not be...

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    I'm wondering if you could send me a aliquot of your xCT antibody ab37185.
    Indeed, I would like to test it on fresh samplesthat will beready at the end of the monthand, at the moment, we are not able to make any order (indeed we are changing oursy...

    Read More

    Thank you for contacting Abcam.

    We are unable to send you any free/trail sizes of our antibodies. When your ordering system is reorganized at the end of the month, you will be able to order the antibody and if it is in stock then it will be s...

    Read More

    Multiple bands in addition to specific band,using human primary blood samples.

    Thank you for your call today and for letting us know about the trouble with ab37185. As we discussed, please let me know if the suggestions that we discussed don't improve the results, and I will be happy to help you further. If you have any questions...

    Read More

    Called because he did not receive the email back from technical support when he sent in the questionnaire.

    Thank you for your enquiry. As discussed on the phone, here is the email that was sent in response to your questionnaire from Kate Hayes on December 1st, 2006. Reply from Abcam to your enquiry regarding ab37185 Dear Ilangovan, In response to ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"