Anti-xCT antibody (ab60171)
Key features and details
- Goat polyclonal to xCT
- Suitable for: ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-xCT antibody
See all xCT primary antibodies -
Description
Goat polyclonal to xCT -
Host species
Goat -
Tested applications
Suitable for: ICCmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Dog, Pig, Orangutan -
Immunogen
Synthetic peptide:
C-KGQTQNFKDAFSGRD
, corresponding to internal sequence amino acids 215-229 of Human xCT -
Positive control
- ICC: A549 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab60171 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC |
Use a concentration of 10 µg/ml.
|
Notes |
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ICC
Use a concentration of 10 µg/ml. |
Target
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Function
Sodium-independent, high-affinity exchange of anionic amino acids with high specificity for anionic form of cystine and glutamate. -
Sequence similarities
Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 483821 Dog
- Entrez Gene: 23657 Human
- Entrez Gene: 26570 Mouse
- Entrez Gene: 100172659 Orangutan
- Entrez Gene: 100049683 Pig
- Omim: 607933 Human
- SwissProt: Q9UPY5 Human
- SwissProt: Q9WTR6 Mouse
see all -
Form
xCT has a predicted molecular weight of 55 kDa; however it has a high number of hydrophobic residues which may affect the migration of the protein in SDS-PAGE. Endogenous monomeric xCT is expected to migrate at ~35 kDa and modified-xCT is expected to migrate at ~55 kDa (PMID: 17035536) -
Alternative names
- Amino acid transport system xc xCT antibody antibody
- Amino acid transport system xc- antibody
- Calcium channel blocker resistance protein CCBR1 antibody
see all
Images
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Immunocytochemistry analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr at 10 ug/mL followed by Alexa Fluor 488 secondary antibody at 2 ug/mL, showing vesicle/ER staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG at 10ug/mL followed by Alexa Fluor 488 secondary antibody at 2ug/mL.
Datasheets and documents
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Datasheet download
References (10)
ab60171 has been referenced in 10 publications.
- Sun S et al. RNA binding protein NKAP protects glioblastoma cells from ferroptosis by promoting SLC7A11 mRNA splicing in an m6A-dependent manner. Cell Death Dis 13:73 (2022). PubMed: 35064112
- Xu X et al. Polyphenols Sourced from Ilex latifolia Thunb. Relieve Intestinal Injury via Modulating Ferroptosis in Weanling Piglets under Oxidative Stress. Antioxidants (Basel) 11:N/A (2022). PubMed: 35624829
- Lofthouse EM et al. N-acetylcysteine, xCT and suppression of Maxi-chloride channel activity in human placenta. Placenta 110:46-55 (2021). PubMed: 34120018
- Umans RA et al. Transcriptional Regulation of Amino Acid Transport in Glioblastoma Multiforme. Cancers (Basel) 13:N/A (2021). PubMed: 34944790
- He P et al. Holly (Ilex latifolia Thunb.) Polyphenols Extracts Alleviate Hepatic Damage by Regulating Ferroptosis Following Diquat Challenge in a Piglet Model. Front Nutr 7:604328 (2020). PubMed: 33385007
- Chen Y et al. Dihydroartemisinin-induced unfolded protein response feedback attenuates ferroptosis via PERK/ATF4/HSPA5 pathway in glioma cells. J Exp Clin Cancer Res 38:402 (2019). PubMed: 31519193
- Ge C et al. The down-regulation of SLC7A11 enhances ROS induced P-gp over-expression and drug resistance in MCF-7 breast cancer cells. Sci Rep 7:3791 (2017). WB ; Human . PubMed: 28630426
- Ye JL et al. EAAT3 promotes amino acid transport and proliferation of porcine intestinal epithelial cells. Oncotarget 7:38681-38692 (2016). WB . PubMed: 27231847
- Chintala S et al. Downregulation of Cystine Transporter x(c) by Irinotecan in human head and neck cancer FaDu xenografts. Chemotherapy 56:223-33 (2010). PubMed: 20551639
- Olm E et al. Extracellular thiol-assisted selenium uptake dependent on the x(c)- cystine transporter explains the cancer-specific cytotoxicity of selenite. Proc Natl Acad Sci U S A 106:11400-5 (2009). WB ; Human . PubMed: 19549867