Overview

  • Product nameAnti-XIAP antibody [2F1]
    See all XIAP primary antibodies
  • Description
    Mouse monoclonal [2F1] to XIAP
  • Tested applicationsSuitable for: Flow Cyt, WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Fusion protein with Human XIAP.

  • Epitopeab28151 reacts with an epitope located in the C terminal region encoded by aa 352/449 of XIAP.
  • Positive control
    • HeLa Cell Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab28151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 22039248

Target

  • FunctionApoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. Inhibitor of caspase-3, -7 and -9. Mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B.
  • Tissue specificityUbiquitous, except peripheral blood leukocytes.
  • Involvement in diseaseDefects in XIAP are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2) [MIM:300635]. XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma.
  • Sequence similaritiesBelongs to the IAP family.
    Contains 3 BIR repeats.
    Contains 1 RING-type zinc finger.
  • DomainThe first BIR domain is involved in interaction with TAB1/MAP3K7IP1 and is important for dimerization. The second BIR domain is sufficient to inhibit caspase-3 and caspase-7, while the third BIR is involved in caspase-9 inhibition. The interactions with SMAC and PRSS25 are mediated by the second and third BIR domains.
  • Post-translational
    modifications
    Ubiquitinated and degraded by the proteasome in apoptotic cells.
    Phosphorylation by PKB/AKT protects XIAP against ubiquitination and protects the protein against proteasomal degradation.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • AP 13 antibody
    • API3 antibody
    • Apoptosis Inhibitor 3 antibody
    • Baculoviral IAP repeat containing 4 antibody
    • Baculoviral IAP Repeat Containing Protein 4 antibody
    • Baculoviral IAP repeat-containing protein 4 antibody
    • BIRC 4 antibody
    • BIRC4 antibody
    • E3 ubiquitin-protein ligase XIAP antibody
    • hIAP-3 antibody
    • hIAP3 antibody
    • HILP antibody
    • IAP 3 antibody
    • IAP like protein antibody
    • IAP-3 antibody
    • IAP-like protein antibody
    • IAP3 antibody
    • ILP 1 antibody
    • ILP antibody
    • ILP1 antibody
    • Inhibitor of apoptosis protein 3 antibody
    • Inhibitor of Apoptosis X Linked antibody
    • Mammalian IAP Homologue A antibody
    • MIHA antibody
    • X linked IAP antibody
    • X linked inhibitor of apoptosis antibody
    • X linked inhibitor of apoptosis E3 ubiquitin protein ligase antibody
    • X linked inhibitor of apoptosis protein antibody
    • X-linked IAP antibody
    • X-linked inhibitor of apoptosis protein antibody
    • Xiap antibody
    • XIAP_HUMAN antibody
    • XLP2 antibody
    see all

Anti-XIAP antibody [2F1] images

  • ab28151 staining XIAP in Mouse colonic epithelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.01% Triton X-100 and blocked with 1% BSA for 45 minutes at 20°C. Samples were incubated with primary antibody (1/50 in 1% BSA + 0.01% Triton X-100) for 2 hours at 22°C. An Alexa Fluor® 488-conjugated Chicken anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Overlay histogram showing HeLA cells stained with ab28151 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab28151, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-XIAP antibody [2F1] (ab28151)

This product has been referenced in:
  • Yi JM  et al. Dual targeting of microtubule and topoisomerase II by a-carboline derivative YCH337 for tumor proliferation and growth inhibition. Oncotarget 6:8960-73 (2015). Human . Read more (PubMed: 25840421) »
  • Leiphrakpam PD  et al. In vivo analysis of insulin-like growth factor type 1 receptor humanized monoclonal antibody MK-0646 and small molecule kinase inhibitor OSI-906 in colorectal cancer. Oncol Rep 31:87-94 (2014). Read more (PubMed: 24173770) »

See all 11 Publications for this product

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (Hep G2)
Specification Hep G2
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Dec 26 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 20°C
Sample Mouse Cell (colonic epithelial cells)
Specification colonic epithelial cells
Permeabilization Yes - Triton 0.01%
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 25 2013

Thank you for your patience.

I have heard back regarding ab28151:

The antibodyhas only been tested in WB with the following lysates: Jurkat, Raji, HeLa, WR19L and PC12 cells.Other samples were not tested.
Also, the crossreacti...

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Thank you for contacting us.

I have contacted the lab regarding the 2 antibodies, and will let you know the informationI receive.

As for the conjugation kits and purification kits, the link is: http://www.abcam.com/Easylink
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Thank you for your reply. I recommended the Protein G beads because they bind more strongly to mouse IgG1 than Protein A does. The image you sent does seem to show the expected 50 kDa band in most of your samples when the Caspase 3 IP was probed with a...

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Thank you for contacting us. I am sorry that this antibody is not working in IP. Could you please provide some details about how your samples were prepared and what buffers were used? What type of beads did you use? I would recommend using Protein G be...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (human islet lysate)
Loading amount 20 µg
Specification human islet lysate
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Nov 17 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (MCF-7)
Loading amount 5 µg
Specification MCF-7
Treatment CDK7 inhibitors
Gel Running Conditions Reduced Denaturing (10)
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Dean Heathcote

Verified customer

Submitted Jun 11 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (primary keratinocytes and HT1080 fibrosarcoma)
Loading amount 60 µg
Specification primary keratinocytes and HT1080 fibrosarcoma
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Nov 05 2007

Thank you for your enquiry. The protein should be ~54 kDa. Please let me know if I can be of further assistance.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"