Overview

  • Product nameAnti-XIAP antibody
    See all XIAP primary antibodies
  • Description
    Rabbit polyclonal to XIAP
  • SpecificityThis antibody detects the 53 kDa human XIAP protein. It does not cross-react with other apoptosis inhibitors.
  • Tested applicationsSuitable for: IHC (PFA fixed), WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Available on request. (Peptide available as ab38275.)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab2541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC (PFA fixed)
WB
ICC/IF
  • Application notesICC/IF: 1/200.
    Immunohistochemistry (PFA fixed): Use at an assay dependent dilution (See PMID: 17094439).
    WB: Use at a concentration of 1.0 µg/ml.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionApoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. Inhibitor of caspase-3, -7 and -9. Mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B.
    • Tissue specificityUbiquitous, except peripheral blood leukocytes.
    • Involvement in diseaseDefects in XIAP are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2) [MIM:300635]. XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma.
    • Sequence similaritiesBelongs to the IAP family.
      Contains 3 BIR repeats.
      Contains 1 RING-type zinc finger.
    • DomainThe first BIR domain is involved in interaction with TAB1/MAP3K7IP1 and is important for dimerization. The second BIR domain is sufficient to inhibit caspase-3 and caspase-7, while the third BIR is involved in caspase-9 inhibition. The interactions with SMAC and PRSS25 are mediated by the second and third BIR domains.
    • Post-translational
      modifications
      Ubiquitinated and degraded by the proteasome in apoptotic cells.
      Phosphorylation by PKB/AKT protects XIAP against ubiquitination and protects the protein against proteasomal degradation.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • AP 13 antibody
      • API3 antibody
      • Apoptosis Inhibitor 3 antibody
      • Baculoviral IAP repeat containing 4 antibody
      • Baculoviral IAP Repeat Containing Protein 4 antibody
      • Baculoviral IAP repeat-containing protein 4 antibody
      • BIRC 4 antibody
      • BIRC4 antibody
      • E3 ubiquitin-protein ligase XIAP antibody
      • hIAP-3 antibody
      • hIAP3 antibody
      • HILP antibody
      • IAP 3 antibody
      • IAP like protein antibody
      • IAP-3 antibody
      • IAP-like protein antibody
      • IAP3 antibody
      • ILP 1 antibody
      • ILP antibody
      • ILP1 antibody
      • Inhibitor of apoptosis protein 3 antibody
      • Inhibitor of Apoptosis X Linked antibody
      • Mammalian IAP Homologue A antibody
      • MIHA antibody
      • X linked IAP antibody
      • X linked inhibitor of apoptosis antibody
      • X linked inhibitor of apoptosis E3 ubiquitin protein ligase antibody
      • X linked inhibitor of apoptosis protein antibody
      • X-linked IAP antibody
      • X-linked inhibitor of apoptosis protein antibody
      • Xiap antibody
      • XIAP_HUMAN antibody
      • XLP2 antibody
      see all

    Anti-XIAP antibody images

    • ab2541 (1/200) staining XIAP in HeLa cells (green). Cells were permeabilised with 0.5% Triton-X100/ PBS, fixed in methanol (-20oC, 10min) and counterstained with DAPI (red) in order to highlight the nucleus. Please refer to abreview for further experimental details.

      See Abreview

    • All lanes : Anti-XIAP antibody (ab2541) at 1 µg/ml

      Lane 1 : Human brain tissue lysate - total protein (ab29466)
      Lane 2 : Human placenta tissue lysate - total protein (ab29745)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 54 kDa
      Observed band size : 54 kDa


      Exposure time : 12 minutes
    • ab2541 staining XIAP in MCF7 cells treated with forskolin (ab120058), by ICC/IF. Increase in XIAP expression correlates with increased concentration of forskolin, as described in literature.
      The cells were incubated at 37°C for 2h in media containing different concentrations of ab120058 (forskolin) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab2541 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.

    References for Anti-XIAP antibody (ab2541)

    This product has been referenced in:
    • Hsu KH  et al. Anti-apoptotic effects of osteopontin through the up-regulation of Mcl-1 in gastrointestinal stromal tumors. World J Surg Oncol 12:189 (2014). WB ; Human . Read more (PubMed: 24947165) »
    • Lopergolo A  et al. Apollon gene silencing induces apoptosis in breast cancer cells through p53 stabilisation and caspase-3 activation. Br J Cancer 100:739-46 (2009). WB ; Human . Read more (PubMed: 19223905) »

    See all 3 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Methanol
    Permeabilization Yes - 0.5% Triton-X100 in PBS
    Username

    Dr. Kirk McManus

    Verified customer

    Submitted Nov 30 2007

    Thank you for your enquiry. I am sorry to hear that your customer has been having difficulties with this antibody. Initially I would like to recommend that they apply the antibody at a dilution of 1:100 at 4oC overnight using 3% BSA/TBST as a blocki...

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    We have been informed by the source of ab2541 that western blots showed mouse intestine was positive for XIAP. We do not have a negative tissue sample available unfortunately. I hope this information will be useful, please do not hesitate to contact...

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    I'm sorry to hear you are having problems with ab2541 in IHC. As we have not tested the antibody in this application it is very difficult to find out what the problem might be and I am waiting to hear back from the source of the antibody regarding ...

    Read More

    This antibody has not been tested on rat or IP so we have no further information on this.

    The immunogen is a 12 a.a. peptide around residue 240 of human XIAP. However, we are unable to disclose the detail sequence, as it is proprietary.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"