Anti-XIAP (phospho S87) antibody (ab193315)

Overview

  • Product name
    Anti-XIAP (phospho S87) antibody
    See all XIAP primary antibodies
  • Description
    Rabbit polyclonal to XIAP (phospho S87)
  • Host species
    Rabbit
  • Specificity
    ab193315 detects endogenous level of XIAP only when phosphorylated at serine 87.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human XIAP (N terminal) (phospho S87) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. [Peptide sequence around phosphorylation site of serine 87(K-V-S(p)-P-N)].
    Database link: P98170

  • Positive control
    • HepG2 cell extract treated with anisomycin.

Properties

Applications

Our Abpromise guarantee covers the use of ab193315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 57 kDa.

Target

  • Function
    Apoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. Inhibitor of caspase-3, -7 and -9. Mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B.
  • Tissue specificity
    Ubiquitous, except peripheral blood leukocytes.
  • Involvement in disease
    Defects in XIAP are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2) [MIM:300635]. XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma.
  • Sequence similarities
    Belongs to the IAP family.
    Contains 3 BIR repeats.
    Contains 1 RING-type zinc finger.
  • Domain
    The first BIR domain is involved in interaction with TAB1/MAP3K7IP1 and is important for dimerization. The second BIR domain is sufficient to inhibit caspase-3 and caspase-7, while the third BIR is involved in caspase-9 inhibition. The interactions with SMAC and PRSS25 are mediated by the second and third BIR domains.
  • Post-translational
    modifications
    Ubiquitinated and degraded by the proteasome in apoptotic cells.
    Phosphorylation by PKB/AKT protects XIAP against ubiquitination and protects the protein against proteasomal degradation.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • AP 13 antibody
    • API3 antibody
    • Apoptosis Inhibitor 3 antibody
    • Baculoviral IAP repeat containing 4 antibody
    • Baculoviral IAP Repeat Containing Protein 4 antibody
    • Baculoviral IAP repeat-containing protein 4 antibody
    • BIRC 4 antibody
    • BIRC4 antibody
    • E3 ubiquitin-protein ligase XIAP antibody
    • hIAP-3 antibody
    • hIAP3 antibody
    • HILP antibody
    • IAP 3 antibody
    • IAP like protein antibody
    • IAP-3 antibody
    • IAP-like protein antibody
    • IAP3 antibody
    • ILP 1 antibody
    • ILP antibody
    • ILP1 antibody
    • Inhibitor of apoptosis protein 3 antibody
    • Inhibitor of Apoptosis X Linked antibody
    • Mammalian IAP Homologue A antibody
    • MIHA antibody
    • X linked IAP antibody
    • X linked inhibitor of apoptosis antibody
    • X linked inhibitor of apoptosis E3 ubiquitin protein ligase antibody
    • X linked inhibitor of apoptosis protein antibody
    • X-linked IAP antibody
    • X-linked inhibitor of apoptosis protein antibody
    • Xiap antibody
    • XIAP_HUMAN antibody
    • XLP2 antibody
    see all

Images

  • All lanes : Anti-XIAP (phospho S87) antibody (ab193315) at 1/500 dilution

    Lane 1 : HepG2 cell extract treated with anisomycin
    Lane 2 : HepG2 cell extract treated with anisomycin with antigen-specific peptide

    Predicted band size: 57 kDa

References

ab193315 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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