Overview

  • Product nameAnti-XK antibody
    See all XK primary antibodies
  • Description
    Rabbit polyclonal to XK
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Cat, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 72-121 (LHLLQLGPLF RCFEVFCIYF QSGNNEEPYV SITKKRQMPK NGLSEEIEKE) of Human XK (NP_066569)

  • Positive control
    • MCF7 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay 1/12500.

Target

  • FunctionMay be involved in sodium-dependent transport of neutral amino acids or oligopeptides.
  • Tissue specificityHigh levels in skeletal muscle, heart, brain, and pancreas; low levels in placenta, lung, liver, and kidney.
  • Involvement in diseaseDefects in XK are the cause of McLeod syndrome (MLS) [MIM:300842]. It is an X-linked multisystem disorder characterized by late onset abnormalities in the neuromuscular and hematopoietic systems.
  • Sequence similaritiesBelongs to the XK family.
  • Post-translational
    modifications
    Not glycosylated.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Kell complex 37 kDa component antibody
    • Kx antigen antibody
    • Membrane transport protein XK antibody
    • XK antibody
    • XK related protein 1 antibody
    • XK-related protein 1 antibody
    • XK_HUMAN antibody
    • XKR1 antibody
    • XRG1 antibody
    see all

Anti-XK antibody images

  • Anti-XK antibody (ab82686) at 1 µg/ml + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 51 kDa
    Observed band size : 51 kDa
    Additional bands at : 25 kDa,31 kDa. We are unsure as to the identity of these extra bands.

References for Anti-XK antibody (ab82686)

ab82686 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"