Overview

  • Product nameAnti-XLF antibody
    See all XLF primary antibodies
  • Description
    Rabbit polyclonal to XLF
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within C-terminal amino acids 267-296 of Human XLF (NP_079058.1).

  • Positive control
    • Hela cell line lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab123443 was purified through a Protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab123443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 33 kDa.

Target

  • FunctionDNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. It may act in concert with XRCC6/XRCC5 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are noncomplementary or partially complementary.
  • Tissue specificityUbiquitously expressed.
  • Involvement in diseaseDefects in NHEJ1 are the cause of severe combined immunodeficiency due to NHEJ1 deficiency (NHEJ1-SCID) [MIM:611291]; also known as autosomal recessive T cell-negative, B cell-negative, NK cell-positive, severe combined immunodeficiency with microcephaly, growth retardation and sensitivity to ionizing radiation or NHEJ1 syndrome. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. NHEJ1-SCID is characterized by a profound T- and B-lymphocytopenia associated with increased cellular sensitivity to ionizing radiation, microcephaly and growth retardation. Some patients may manifest SCID with sensitivity to ionizing radiation without microcephaly and mild growth retardation, probably due to hypomorphic NHEJ1 mutations.
    Note=A chromosomal aberration involving NHEJ1 is found in a patient with polymicrogyria. Translocation t(2;7)(q35;p22).
  • Sequence similaritiesBelongs to the XLF family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cernunnos antibody
    • FLJ12610 antibody
    • NHEJ 1 antibody
    • Nhej1 antibody
    • NHEJ1, S. cerevisiae, homolog of antibody
    • NHEJ1_HUMAN antibody
    • Non homologous end joining factor 1 antibody
    • Non-homologous end-joining factor 1 antibody
    • Nonhomologous end joining factor 1 antibody
    • OTTHUMP00000164168 antibody
    • OTTHUMP00000206275 antibody
    • OTTHUMP00000206279 antibody
    • Protein cernunnos antibody
    • XLF antibody
    • XRCC4 like factor antibody
    • XRCC4-like factor antibody
    see all

Anti-XLF antibody images

  • Anti-XLF antibody (ab123443) at 1/100 dilution + Hela cell line lysate at 35 µg

    Predicted band size : 33 kDa

References for Anti-XLF antibody (ab123443)

ab123443 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab123443.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"