Anti-XP32 antibody (ab122672)
Key features and details
- Rabbit polyclonal to XP32
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-XP32 antibody -
Description
Rabbit polyclonal to XP32 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human XP32 aa 150-250.
Database link: Q5T750 -
Positive control
- Human liver tissue; Human plasma.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122672 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/250 - 1/500.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
Notes |
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WB
1/250 - 1/500. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
Target
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Tissue specificity
Expressed at high levels in normal and psoriatic skin, but not in normal keratinocytes, A431 cells, or any of the other cell lines or tissues tested. - Information by UniProt
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Database links
- Entrez Gene: 100129271 Human
- SwissProt: Q5T750 Human
- Unigene: 601077 Human
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Alternative names
- Chromosome 1 open reading frame 68 antibody
- LEP7 antibody
- Skin-specific protein 32 antibody
see all
Images
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Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab122672 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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ab122672, at 1/50 dilution, staining XP32 in paraffin-embedded Human liver tissue by Immunohistochemistry.
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All lanes : Anti-XP32 antibody (ab122672) at 1/250 dilution
Lane 1 : RT 4 lysate
Lane 2 : U 251 MG lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab122672 has not yet been referenced specifically in any publications.