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Rabbit polyclonal to XP32
antigen sequence: PCSTSYCCLA PRTFGVSPLR RWIQRPQNCN TGSSGCCENS GSSGCCGSGG CGCSCGCGSS G, corresponding to amino acids 165-225 of Human XP32.
- Human liver tissue; Human plasma.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/250 - 1/500.
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Expressed at high levels in normal and psoriatic skin, but not in normal keratinocytes, A431 cells, or any of the other cell lines or tissues tested.
Information by UniProt
- Chromosome 1 open reading frame 68 antibody
- LEP7 antibody
- Skin-specific protein 32 antibody
Immunocytochemistry/ Immunofluorescence - Anti-XP32 antibody (ab122672)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab122672 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XP32 antibody (ab122672)
ab122672, at 1/50 dilution, staining XP32 in paraffin-embedded Human liver tissue by Immunohistochemistry.
Western blot - Anti-XP32 antibody (ab122672)
All lanes : Anti-XP32 antibody (ab122672) at 1/250 dilution
Lane 1 : RT 4 lysate
Lane 2 : U 251 MG lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"