Overview

  • Product nameAnti-XPA antibody [5F12]
    See all XPA primary antibodies
  • Description
    Mouse monoclonal [5F12] to XPA
  • Tested applicationsSuitable for: ELISA, WB, Inhibition Assay, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant full length XPA protein (Human)

  • EpitopeAmino acids 30-47.
  • Positive control
    • Crude extract of HeLa cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab65963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 31 kDa).
Inhibition Assay Use at an assay dependent concentration. Inhibition of in vitro excision repair reaction and inhibition of XPA interaction with ERCC1 and TFIIH.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionInvolved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation.
  • Tissue specificityExpressed in various cell lines and in skin fibroblasts.
  • Involvement in diseaseDefects in XPA are a cause of xeroderma pigmentosum complementation group A (XP-A) [MIM:278700]; also known as xeroderma pigmentosum type 1 (XP1). XP-A is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Group A patients show the most severe skin symptoms and progressive neurological disorders.
  • Sequence similaritiesBelongs to the XPA family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated by HERC2 leading to degradation by the proteasome.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA repair protein complementing XP A cells antibody
    • DNA repair protein complementing XP-A cells antibody
    • DNA repair protein complementing XPA cells antibody
    • Excision repair controlling antibody
    • Xeroderma pigmentosum 1 antibody
    • Xeroderma pigmentosum complementation group A antibody
    • Xeroderma pigmentosum group A complementing protein antibody
    • Xeroderma pigmentosum group A-complementing protein antibody
    • XP 1 antibody
    • XP1 antibody
    • xpa antibody
    • XPA_HUMAN antibody
    • Xpac antibody
    see all

Anti-XPA antibody [5F12] images

  • Immunofluorescent staining of human fibroblast cells (GM0637) using ab65963 at a dilution of 1/100. The cells were non-irradiated (left) or irradiated with UV at 20 J/m2 (right), fixed after 30 minutes with paraformaldehyde and counter-stained with Hoescht. The top panels show the antibody staining, the middle panels show the Hoescht staining and the bottom panels are a merge of the two images. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-mouse IgG, used at a dilution of 1/5000.

  • Anti-XPA antibody [5F12] (ab65963) at 0.1 µg/ml + Crude extract of HeLa cells at 200 µg

    Predicted band size : 31 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

References for Anti-XPA antibody [5F12] (ab65963)

This product has been referenced in:
  • Sousa MM  et al. An inverse switch in DNA base excision and strand break repair contributes to melphalan resistance in multiple myeloma cells. PLoS One 8:e55493 (2013). WB ; Human . Read more (PubMed: 23405159) »
  • Herrera M  et al. Differences in repair of DNA cross-links between lymphocytes and epithelial tumor cells from colon cancer patients measured in vitro with the comet assay. Clin Cancer Res 15:5466-72 (2009). WB ; Human . Read more (PubMed: 19690199) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"