Overview

  • Product name
  • Description
    Rabbit polyclonal to XPA
  • Specificity
    ab58464 detects endogenous levels of total XPA protein.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from internal sequence of human XPA.

  • Positive control
    • Extracts from COLO205 cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab58464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 31 kDa).
ELISA 1/5000.

Target

  • Function
    Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation.
  • Tissue specificity
    Expressed in various cell lines and in skin fibroblasts.
  • Involvement in disease
    Defects in XPA are a cause of xeroderma pigmentosum complementation group A (XP-A) [MIM:278700]; also known as xeroderma pigmentosum type 1 (XP1). XP-A is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Group A patients show the most severe skin symptoms and progressive neurological disorders.
  • Sequence similarities
    Belongs to the XPA family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated by HERC2 leading to degradation by the proteasome.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA repair protein complementing XP A cells antibody
    • DNA repair protein complementing XP-A cells antibody
    • DNA repair protein complementing XPA cells antibody
    • Excision repair controlling antibody
    • Xeroderma pigmentosum 1 antibody
    • Xeroderma pigmentosum complementation group A antibody
    • Xeroderma pigmentosum group A complementing protein antibody
    • Xeroderma pigmentosum group A-complementing protein antibody
    • XP 1 antibody
    • XP1 antibody
    • xpa antibody
    • XPA_HUMAN antibody
    • Xpac antibody
    see all

Images

  • All lanes : Anti-XPA antibody (ab58464) at 1/500 dilution

    Lane 1 : Extracts from COLO205 cells
    Lane 2 : Extracts from COLO205 cells with immunizing peptide


    Predicted band size : 31 kDa
    Observed band size : 41 kDa (why is the actual band size different from the predicted?)

References

ab58464 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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