Anti-XPA antibody (ab85914)
Key features and details
- Rabbit polyclonal to XPA
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-XPA antibody
See all XPA primary antibodies -
Description
Rabbit polyclonal to XPA -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rhesus monkey, Gorilla, Elephant -
Immunogen
Synthetic peptide, corresponding to a region between residues 1 and 50 of Human XPA (NP_000371.1)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab85914 was affinity purified using an epitope specific to XPA immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85914 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/2000 - 1/10000. Detects a band of approximately 39 kDa (predicted molecular weight: 31 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 39 kDa (predicted molecular weight: 31 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation. -
Tissue specificity
Expressed in various cell lines and in skin fibroblasts. -
Involvement in disease
Defects in XPA are a cause of xeroderma pigmentosum complementation group A (XP-A) [MIM:278700]; also known as xeroderma pigmentosum type 1 (XP1). XP-A is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Group A patients show the most severe skin symptoms and progressive neurological disorders. -
Sequence similarities
Belongs to the XPA family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated by HERC2 leading to degradation by the proteasome. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7507 Human
- Omim: 611153 Human
- SwissProt: P23025 Human
- Unigene: 654364 Human
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Alternative names
- DNA repair protein complementing XP A cells antibody
- DNA repair protein complementing XP-A cells antibody
- DNA repair protein complementing XPA cells antibody
see all
Images
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All lanes : Anti-XPA antibody (ab85914) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 31 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Detection: Chemiluminescence with exposure time of 30 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling XPA with ab85914 at 1/1000 (0.2µg/ml). Detection: DAB.
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Detection of XPA by Western Blot of Immunprecipitate.
ab85914 at 0.1µg/ml staining XPA in HeLa whole cell lysate immunoprecipitated using ab85914 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab85914 has been referenced in 8 publications.
- Nazlamova L et al. Microtubule modification defects underlie cilium degeneration in cell models of retinitis pigmentosa associated with pre-mRNA splicing factor mutations. Front Genet 13:1009430 (2022). PubMed: 36176300
- Zhang Y et al. Idarubicin combats abiraterone and enzalutamide resistance in prostate cells via targeting XPA protein. Cell Death Dis 13:1034 (2022). PubMed: 36509750
- McKinney JA et al. Distinct DNA repair pathways cause genomic instability at alternative DNA structures. Nat Commun 11:236 (2020). PubMed: 31932649
- Zhu B et al. The protective role of DOT1L in UV-induced melanomagenesis. Nat Commun 9:259 (2018). PubMed: 29343685
- Ding X et al. Differential sensitivities of cellular XPA and PARP-1 to arsenite inhibition and zinc rescue. Toxicol Appl Pharmacol 331:108-115 (2017). PubMed: 28552776
- Zhou X et al. Differential binding of monomethylarsonous acid compared to arsenite and arsenic trioxide with zinc finger peptides and proteins. Chem Res Toxicol 27:690-8 (2014). IP ; Human . PubMed: 24611629
- King BS et al. Poly(ADP-ribose) contributes to an association between poly(ADP-ribose) polymerase-1 and xeroderma pigmentosum complementation group A in nucleotide excision repair. J Biol Chem 287:39824-33 (2012). IP ; Human . PubMed: 23038248
- Zhou X et al. Arsenite Interacts Selectively with Zinc Finger Proteins Containing C3H1 or C4 Motifs. J Biol Chem 286:22855-63 (2011). IP ; Human . PubMed: 21550982