Overview

  • Product nameAnti-XPB antibody
    See all XPB primary antibodies
  • Description
    Rabbit polyclonal to XPB
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Drosophila melanogaster, Zebrafish, Cynomolgus Monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human XPB.

    (Peptide available as ab30634.)

  • Positive control
    • ab27317 gave a positive result in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) MEF1 (Mouse embryonic fibroblast cell line) PC12 (Rat adrenal pheochromocytoma cell line)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab27317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 98, 60 kDa (predicted molecular weight: 89 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionATP-dependent 3'-5' DNA helicase, component of the core-TFIIH basal transcription factor, involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. Acts by opening DNA either around the RNA transcription start site or the DNA damage.
  • Involvement in diseaseDefects in ERCC3 are the cause of xeroderma pigmentosum complementation group B (XP-B) [MIM:610651]; also known as xeroderma pigmentosum II (XP2) or XP group B (XPB) or xeroderma pigmentosum group B combined with Cockayne syndrome (XP-B/CS). Xeroderma pigmentosum is an autosomal recessive pigmentary skin disorder characterized by solar hypersensitivity of the skin, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Some XP-B patients present features of Cockayne syndrome, including dwarfism, sensorineural deafness, microcephaly, mental retardation, pigmentary retinopathy, ataxia, decreased nerve conduction velocities.
    Defects in ERCC3 are a cause of trichothiodystrophy photosensitive (TTDP) [MIM:601675]. TTDP is an autosomal recessive disease characterized by sulfur-deficient brittle hair and nails, ichthyosis, mental retardation, impaired sexual development, abnormal facies and cutaneous photosensitivity correlated with a nucleotide excision repair (NER) defect. Neonates with trichothiodystrophy and ichthyosis are usually born with a collodion membrane. The severity of the ichthyosis after the membrane is shed is variable, ranging from a mild to severe lamellar ichthyotic phenotype. There are no reports of skin cancer associated with TTDP.
  • Sequence similaritiesBelongs to the helicase family. RAD25/XPB subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Basic transcription factor 2 89 kDa subunit antibody
    • BTF 2 antibody
    • BTF2 antibody
    • BTF2 p89 antibody
    • DNA excision repair protein ERCC-3 antibody
    • DNA repair protein complementing XP-B cells antibody
    • ERCC 3 antibody
    • ercc3 antibody
    • ERCC3_HUMAN antibody
    • Excision Repair Cross-complementing Rodent Repair deficiency complementation Group 3 antibody
    • GTF2H antibody
    • RAD 25 antibody
    • RAD25 antibody
    • TFIIH 89 kDa subunit antibody
    • TFIIH antibody
    • TFIIH basal transcription factor complex 89 kDa subunit antibody
    • TFIIH basal transcription factor complex helicase XPB subunit antibody
    • TFIIH p89 antibody
    • Xeroderma pigmentosum group B-complementing protein antibody
    see all

Anti-XPB antibody images

  • All lanes : Anti-XPB antibody (ab27317) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
    Lane 2 : Jurkat whole cell lysate (ab7899) at 20 µg

    Secondary
    IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 89 kDa
    Observed band size : 98 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-XPB antibody (ab27317) at 1 µg/ml

    Lane 1 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 2 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 89 kDa
    Observed band size : 98 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab27317 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27317, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-XPB antibody (ab27317)

This product has been referenced in:
  • Pannucci NL  et al. Loss of the xeroderma pigmentosum group B protein binding site impairs p210 BCR/ABL1 leukemogenic activity. Blood Cancer J 3:e135 (2013). WB . Read more (PubMed: 23955590) »

See 1 Publication for this product

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