Overview

  • Product name
    Anti-XPG antibody [8H7]
    See all XPG primary antibodies
  • Description
    Mouse monoclonal [8H7] to XPG
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human XPG (Xeroderma Pigmentosum type G) protein produced in Baculovirus.

  • Epitope
    8H7 binds between human XPG residues Ser 947 and Ala 1165.
  • General notes
    Works well on crude cell extract.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Phosphate buffered saline
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    8H7
  • Myeloma
    Sp2
  • Isotype
    IgG2a
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab46 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/625 - 1/1250. Predicted molecular weight: 133 kDa.
IP Use at 2 µg/mg of lysate.

Target

  • Function
    Single-stranded structure-specific DNA endonuclease involved in DNA excision repair. Makes the 3'incision in DNA nucleotide excision repair (NER). Acts as a cofactor for a DNA glycosylase that removes oxidized pyrimidines from DNA. May also be involved in transcription-coupled repair of this kind of damage, in transcription by RNA polymerase II, and perhaps in other processes too.
  • Involvement in disease
    Defects in ERCC5 are the cause of xeroderma pigmentosum complementation group G (XP-G) [MIM:278780]; also known as xeroderma pigmentosum VII (XP7). Xeroderma pigmentosum is an autosomal recessive pigmentary skin disorder characterized by solar hypersensitivity of the skin, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Some XP-G patients present features of Cockayne syndrome, including dwarfism, sensorineural deafness, microcephaly, mental retardation, pigmentary retinopathy, ataxia, decreased nerve conduction velocities.
  • Sequence similarities
    Belongs to the XPG/RAD2 endonuclease family. XPG subfamily.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • COFS 3 antibody
    • COFS3 antibody
    • DNA excision repair protein ERCC 5 antibody
    • DNA excision repair protein ERCC-5 antibody
    • DNA excision repair protein ERCC5 antibody
    • DNA repair protein complementing XP G cells antibody
    • DNA repair protein complementing XP-G cells antibody
    • DNA repair protein complementing XPG cells antibody
    • ERCC 5 antibody
    • ERCC5 antibody
    • ERCC5_HUMAN antibody
    • ERCM 2 antibody
    • ERCM2 antibody
    • Excision repair cross complementation group 5 antibody
    • Excision Repair Cross Complementing Rodent Repair Deficiency antibody
    • Excision repair cross complementing rodent repair deficiency complementation group 5 antibody
    • Excision repair protein antibody
    • OTTHUMP00000064902 antibody
    • UVDR antibody
    • Xeroderma Pigmentosum Complementation Group G antibody
    • Xeroderma pigmentosum complementation group G protein antibody
    • Xeroderma pigmentosum group G complementing protein antibody
    • Xeroderma pigmentosum group G-complementing protein antibody
    • XPG antibody
    • XPG complementing protein antibody
    • XPGC antibody
    see all

References

This product has been referenced in:
  • Trego KS  et al. The DNA repair endonuclease XPG interacts directly and functionally with the WRN helicase defective in Werner syndrome. Cell Cycle 10:1998-2007 (2011). Human . Read more (PubMed: 21558802) »
  • Igoucheva O  et al. Involvement of ERCC1/XPF and XPG in oligodeoxynucleotide-directed gene modification. Oligonucleotides 16:94-104 (2006). WB ; Human . Read more (PubMed: 16584298) »

See all 2 Publications for this product

Customer reviews and Q&As

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa cell)
Total protein in input
700 µg
Specification
HeLa cell
Immuno-precipitation step
Protein A/G
Username

Roberta Guglielmino

Verified customer

Submitted Apr 11 2007

Thank you for your patience. The antibody was used in the following publications which I hope will be of use to you: Evans E et al. 1997. EMBO J. 16:625-638. Constantinou A et al. 1999. J. Biol. Chem. 274:5637-5648. Araujo SJ et al. 2001. Mol. Ce...

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The MW of the the human XPG protein is 185 kDa and clone 8H7 binds between human XPG residues Ser 947 and Ala 1165. If you have any more questions, please contact us again.

I am still waiting for details regarding ab46 and will contact you when I receive them. For ab6264, the originator has informed me that the epitope has not been determined.

This antibody has not been tested on paraffin sections.

This antibody has not yet been tested in mouse.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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