Anti-XPG antibody (ab64931)
Key features and details
- Rabbit polyclonal to XPG
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-XPG antibody
See all XPG primary antibodies -
Description
Rabbit polyclonal to XPG -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide derived from an internal sequence within Human XPG.
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Positive control
- Extracts from K562 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab64931 was affinity-purified from rabbit antiserum by affinity-chromatography using an epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64931 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 133 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 133 kDa). |
Target
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Function
Single-stranded structure-specific DNA endonuclease involved in DNA excision repair. Makes the 3'incision in DNA nucleotide excision repair (NER). Acts as a cofactor for a DNA glycosylase that removes oxidized pyrimidines from DNA. May also be involved in transcription-coupled repair of this kind of damage, in transcription by RNA polymerase II, and perhaps in other processes too. -
Involvement in disease
Defects in ERCC5 are the cause of xeroderma pigmentosum complementation group G (XP-G) [MIM:278780]; also known as xeroderma pigmentosum VII (XP7). Xeroderma pigmentosum is an autosomal recessive pigmentary skin disorder characterized by solar hypersensitivity of the skin, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Some XP-G patients present features of Cockayne syndrome, including dwarfism, sensorineural deafness, microcephaly, mental retardation, pigmentary retinopathy, ataxia, decreased nerve conduction velocities. -
Sequence similarities
Belongs to the XPG/RAD2 endonuclease family. XPG subfamily. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2073 Human
- Omim: 133530 Human
- SwissProt: P28715 Human
- Unigene: 258429 Human
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Alternative names
- COFS 3 antibody
- COFS3 antibody
- DNA excision repair protein ERCC 5 antibody
see all
Images
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All lanes : Anti-XPG antibody (ab64931) at 1/500 dilution
Lane 1 : Extracts from K562 cells
Lane 2 : Extracts from K562 cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 133 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab64931 has been referenced in 1 publication.
- Gaddameedhi S et al. Similar nucleotide excision repair capacity in melanocytes and melanoma cells. Cancer Res 70:4922-30 (2010). WB ; Human . PubMed: 20501836