Overview

  • Product name
    Anti-XRCC1 antibody [33-2-5]
    See all XRCC1 primary antibodies
  • Description
    Mouse monoclonal [33-2-5] to XRCC1
  • Specificity
    This antibody is specific for XRCC1 (85 kDa).
  • Tested applications
    Suitable for: WB, IP, IHC-P, IHC-Fr, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Human, Chinese hamster
  • Immunogen

    Recombinant full length protein corresponding to Human XRCC1.

  • Positive control
    • IHC-P: Human testis tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab1838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 85 kDa.
IP Use at an assay dependent concentration. PubMed: 17982170
IHC-P 1/50 - 1/100. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/50 - 1/100.
ICC/IF 1/200.
Flow Cyt Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.
  • Sequence similarities
    Contains 2 BRCT domains.
  • Post-translational
    modifications
    Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.
    Sumoylated.
  • Cellular localization
    Nucleus. Accumulates at sites of DNA damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA repair protein XRCC1 antibody
    • RCC antibody
    • X ray repair complementing defective repair in chinese hamster antibody
    • X ray Repair Complementing Defective Repair in Chinese Hamster Cells antibody
    • X ray repair complementing defective repair in chinese hamster cells 1 antibody
    • X ray repair cross complementing 1 antibody
    • X ray repair cross complementing protein 1 antibody
    • X ray repair, complementing defective, repair in Chinese hamster antibody
    • X-ray repair cross-complementing protein 1 antibody
    • XRCC 1 antibody
    • Xrcc1 antibody
    • XRCC1_HUMAN antibody
    see all

Anti-XRCC1 antibody [33-2-5] images

  • All lanes : Anti-XRCC1 antibody [33-2-5] (ab1838) at 1/500 dilution

    Lane 1 : Human dermal fibroblast - whole cell lysate. Treated with non-targeting siRNA
    Lane 2 : Human dermal fibroblast - whole cell lysate. Treated with XRCC1 siRNA

    Secondary
    HRP-conjugated rabbit anti-mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 85 kDa
    Observed band size : 85 kDa


    Exposure time : 30 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling XRCC1 with ab1838.

  • ab1838 staining XRCC1 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 4% BSA for 10 minutes. Samples were incubated with primary antibody (1/200) for 1 hour. An undiluted Alexa Fluor® 555-conjugated donkey anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab1838 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1838, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-XRCC1 antibody [33-2-5] (ab1838)

This product has been referenced in:
  • Zhang Y  et al. Prognostic and predictive role of COX-2, XRCC1 and RASSF1 expression in patients with esophageal squamous cell carcinoma receiving radiotherapy. Oncol Lett 13:2549-2556 (2017). IHC-P ; Human . Read more (PubMed: 28454432) »
  • Cinesi C  et al. Contracting CAG/CTG repeats using the CRISPR-Cas9 nickase. Nat Commun 7:13272 (2016). WB . Read more (PubMed: 27827362) »

See all 23 Publications for this product

Product Wall

Application
Western blot
Sample
Mouse Cell lysate - whole cell (mef)
Loading amount
40 µg
Specification
mef
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted May 16 2012

Thank you for your reply.

I am very pleased to hear you would like to accept our offer and test ab1838 in mouse. As requested, I am providing the following testing discount code:

DISCOUNT CODE: #####
Expiration date: ##### Read More

Thank you for your telephone call earlier today.

As discussed on teh phone, as far as we are aware, ab1838 has never been tested with samples from mouse]. All tested species cross-reactivity information is stated on our datasheets, and these...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (U2OS)
Loading amount
25 µg
Specification
U2OS
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Andrew Cobb

Verified customer

Submitted Mar 29 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
30 µg
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing (10% Tris-Gly gel)
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Nov 17 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
5 µg
Specification
HeLa
Gel Running Conditions
Reduced Denaturing (acrylamide 9%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 25 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Dermal Fibroblast)
Loading amount
10000 cells
Specification
Dermal Fibroblast
Treatment
XRCC1 or non-targeting siRNA
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 17 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Dermal Fibroblast)
Specification
Dermal Fibroblast
Fixative
Formaldehyde
Permeabilization
Yes - Triton
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

Submitted Aug 28 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (cervix (Hela))
Specification
cervix (Hela)
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
Username

Dr. Alexander Rapp

Verified customer

Submitted Feb 01 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela)
Specification
Hela
Fixative
Formaldehyde
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 4%
Username

Mr. Oliver Mortusewicz

Verified customer

Submitted Oct 17 2006

1-10 of 13 Abreviews or Q&A

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