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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XRCC1 antibody [33-2-5] (ab1838)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XRCC1 antibody [33-2-5] (ab1838)](/ps/datasheet/images/ab1838_1.jpg)
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Western blot - XRCC1 antibody [33-2-5] (ab1838)
![Western blot - XRCC1 antibody [33-2-5] (ab1838)](/ps/datasheet/images/ab1838_2.jpg)
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Product Name
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XRCC1 antibody [33-2-5]
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See all XRCC1 antibodies (11)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [33-2-5] to XRCC1
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Immunogen
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Recombinant full length protein (Human).
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Reacts with
(species key)
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Hu, Rat, ChHm
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Specificity
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This antibody is specific for XRCC1 (85 kDa).
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Tested applications
(see key)
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ICC/IF, IHC-Fr, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/200 (see Abreview).
IF: Use at an assay dependent dilution.
IHC-P: 1/50 - 1/100. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: 1/50 - 1/100.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 85 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Testis.
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Cancer Metabolism >> Response to hypoxia
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
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Relevance
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XRCC1 is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. In testis XRCC1 is expressed at high levels.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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33-2-5
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Isotype
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IgG2b
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 1% BSA Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this XRCC1 antibody [33-2-5] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for XRCC1 antibody [33-2-5] images, references, products related to ab1838 and other tools.
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XRCC1 antibody [33-2-5] images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XRCC1 antibody [33-2-5] (ab1838)
ab1838 - immunohistochemistry
Formalin fixed paraffin embedded human testis stained with XRCC1, using ABC and AEC chromogen.
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Western blot - XRCC1 antibody [33-2-5] (ab1838)
All lanes : XRCC1 antibody [33-2-5] (ab1838) at 1/500 dilution
Lane 1 : Human dermal fibroblast - whole cell lysate. Treated with non-targeting siRNA
Lane 2 : Human dermal fibroblast - whole cell lysate. Treated with XRCC1 siRNA
Secondary
HRP conjugated rabbit ant-mouse antibody at 1/2500 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 85 kDa
Observed band size : 85 kDa
Exposure time : 30 seconds This image is courtesy of an anonymous Abreview
See Abreview
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References for XRCC1 antibody [33-2-5] (ab1838)
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This product has been used in: (two most recent references)
Sato S et al. Combination chemotherapy of oxaliplatin and 5-fluorouracil may be an effective regimen for mucinous adenocarcinoma of the ovary: a potential treatment strategy. Cancer Sci 100:546-51 (2009). WB. PubMed: 19154404
Das BB et al. Optimal function of the DNA repair enzyme TDP1 requires its phosphorylation by ATM and/or DNA-PK. EMBO J : (2009). WB, ICC/IF; Human. PubMed: 19851285
See all 7 publication references for this product.
If you publish research using ab1838 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to XRCC1
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XRCC1 antibody [33-2-5] - more information
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Customer reviews (feedback) regarding XRCC1 antibody [33-2-5] |
Customer FAQs regarding XRCC1 antibody [33-2-5] |
Protocols for XRCC1 antibody [33-2-5] |
Price and availability of products related to XRCC1 antibody [33-2-5] |
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XRCC1 antibody [33-2-5] - related products:
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XRCC1 antibody [33-2-5] - other tools:
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Contact Abcam with a Technical Enquiry about XRCC1 antibody [33-2-5] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XRCC1 antibody [33-2-5] (ab1838)](/index.html?datasheet=1838#ab1838_1.jpg)
![Western blot - XRCC1 antibody [33-2-5] (ab1838)](/index.html?datasheet=1838#ab1838_2.jpg)
