Anti-XRCC1 antibody [33-2-5] (ab1838)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XRCC1 antibody [33-2-5] (ab1838)

ab1838 - immunohistochemistry

Formalin fixed paraffin embedded human testis stained with XRCC1, using ABC and AEC chromogen.

Western blot - XRCC1 antibody [33-2-5] (ab1838)

All lanes : Anti-XRCC1 antibody [33-2-5] (ab1838) at 1/500 dilution

Lane 1 : Human dermal fibroblast - whole cell lysate. Treated with non-targeting siRNA
Lane 2 : Human dermal fibroblast - whole cell lysate. Treated with XRCC1 siRNA

Secondary
HRP conjugated rabbit ant-mouse antibody at 1/2500 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 85 kDa
Observed band size : 85 kDa


Exposure time : 30 seconds

This image is courtesy of an anonymous Abreview

Flow Cytometry-Anti-XRCC1 antibody [33-2-5](ab1838)

Overlay histogram showing HeLa cells stained with ab1838 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1838, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x10⁶ cells ) used under the same conditions. Acquisition of >5,000 events was performed.