Overview

  • Product nameAnti-XRCC1 antibody
    See all XRCC1 primary antibodies
  • Description
    Rabbit polyclonal to XRCC1
  • Specificityab58465 detects endogenous levels of total XRCC1 protein.
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from internal sequence of human XRCC1.

  • Positive control
    • WB: Extracts from Jurkat cells. IHC-P: human lung carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab58465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.

Target

  • FunctionCorrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.
  • Sequence similaritiesContains 2 BRCT domains.
  • Post-translational
    modifications
    Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.
    Sumoylated.
  • Cellular localizationNucleus. Accumulates at sites of DNA damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA repair protein XRCC1 antibody
    • RCC antibody
    • X ray repair complementing defective repair in chinese hamster antibody
    • X ray Repair Complementing Defective Repair in Chinese Hamster Cells antibody
    • X ray repair complementing defective repair in chinese hamster cells 1 antibody
    • X ray repair cross complementing 1 antibody
    • X ray repair cross complementing protein 1 antibody
    • X ray repair, complementing defective, repair in Chinese hamster antibody
    • X-ray repair cross-complementing protein 1 antibody
    • XRCC 1 antibody
    • Xrcc1 antibody
    • XRCC1_HUMAN antibody
    see all

Anti-XRCC1 antibody images

  • All lanes : Anti-XRCC1 antibody (ab58465) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from Jurkat cells with immunizing peptide


    Predicted band size : 70 kDa
    Observed band size : 70 kDa
  • Ab58465, at 1/50 dilution, staining paraffin embedded human lung carcinoma tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunising peptide.

References for Anti-XRCC1 antibody (ab58465)

ab58465 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"