Overview

  • Product nameAnti-XRCC1 antibody
    See all XRCC1 primary antibodies
  • Description
    Rabbit polyclonal to XRCC1
  • SpecificityThis antibody recognises human XRCC1 (x-ray cross complementing factor -1), a base excision repair protein.
  • Tested applicationsSuitable for: IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Guinea pig, Hamster, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide (Human) conjugated to KLH - which represented aportion within the last 100 amino acids of the human X-ray Repair Cross Complementing Protein 1 (GenBank PID 11425942).

  • General notesRecently XRCC-1 has been shown to be physically associated with other DNA repair enzymes, including PARP, DNA ligase III and DNA polymerase beta.

Properties

Applications

Our Abpromise guarantee covers the use of ab9147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 2 - 6 µg/ml.
WB 1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 70 kDa).
ICC/IF 1/200 - 1/500.

Target

  • FunctionCorrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.
  • Sequence similaritiesContains 2 BRCT domains.
  • Post-translational
    modifications
    Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.
    Sumoylated.
  • Cellular localizationNucleus. Accumulates at sites of DNA damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA repair protein XRCC1 antibody
    • RCC antibody
    • X ray repair complementing defective repair in chinese hamster antibody
    • X ray Repair Complementing Defective Repair in Chinese Hamster Cells antibody
    • X ray repair complementing defective repair in chinese hamster cells 1 antibody
    • X ray repair cross complementing 1 antibody
    • X ray repair cross complementing protein 1 antibody
    • X ray repair, complementing defective, repair in Chinese hamster antibody
    • X-ray repair cross-complementing protein 1 antibody
    • XRCC 1 antibody
    • Xrcc1 antibody
    • XRCC1_HUMAN antibody
    see all

Anti-XRCC1 antibody images

  • All lanes : Anti-XRCC1 antibody (ab9147) at 1 µg/ml

    Lane 1 : Whole cell extract (50mcg) from mock treated HeLa cells.
    Lane 2 : Whole cell extract (50mcg) from siRNA treated HeLa cells.


    Predicted band size : 70 kDa
    Observed band size : 76,90 kDa (why is the actual band size different from the predicted?)
  • ab9147 staining human HeLa cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.5% Triton X100 prior to blocking in 5% BSA for 30 minutes at 20°C.  The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 20°C.  A Cy3® conjugated donkey anti-rabbit antibody diluted 1/400 was used as the secondary.

    See Abreview

References for Anti-XRCC1 antibody (ab9147)

This product has been referenced in:
  • Yousif AS  et al. Differential regulation of S-region hypermutation and class-switch recombination by noncanonical functions of uracil DNA glycosylase. Proc Natl Acad Sci U S A 111:E1016-24 (2014). Read more (PubMed: 24591630) »
  • Slupianek A  et al. BCR/ABL stimulates WRN to promote survival and genomic instability. Cancer Res 71:842-51 (2011). WB ; Mouse . Read more (PubMed: 21123451) »

See all 9 Publications for this product

Product Wall

Application Western blot
Sample Zebrafish Cell lysate - whole cell (Whole embyro 24hpf)
Gel Running Conditions Non-reduced Denaturing (4-20%)
Loading amount 50 µg
Specification Whole embyro 24hpf
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Mr. Stephen Moore

Verified customer

Submitted Dec 04 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cultured Cells (HeLa cells)
Specification HeLa cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Alexander Rapp

Verified customer

Submitted Apr 18 2008

Thank you for the details in which you have provided. Since you got absolutely no signal even with the HeLa nuclear extract (what is recommended as a positive control) it's definitely telling me that something is wrong with the batch/vial that you rece...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"