The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Detects a band of approximately 34 kDa (predicted molecular weight: 32 kDa).
1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Application notesIs unsuitable for ICC,IHC-P or IP.
FunctionInvolved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. The BCDX2 complex binds single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA.
Sequence similaritiesBelongs to the RecA family. RAD51 subfamily.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.