The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: 1/1. Perform enzymatic antigen retrieval before commencing with IHC staining protocol, by boiling tissue sections in 10mM
Citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionInvolved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. The BCDX2 complex binds single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA.
Sequence similaritiesBelongs to the RecA family. RAD51 subfamily.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.