The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and RAD51C.
Belongs to the RecA family. RAD51 subfamily.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Mitochondrion. Accumulates in discrete nuclear foci prior to DNA damage, and these foci persist throughout the time course of DNA repair.
X ray repair complementing defective repair in Chinese hamster antibody
X ray repair complementing defective repair in Chinese hamster cells 3 antibody
X ray repair cross complementing protein 3 antibody
X-ray repair cross-complementing protein 3 antibody
XRCC 3 antibody
Western blot - Anti-XRCC3 antibody (ab103070)
All lanes : Anti-XRCC3 antibody (ab103070) at 1/250 dilution
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate Lane 4 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37 kDa Observed band size: 37 kDa Additional bands at: 24 kDa, 49 kDa, 56 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab103070 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.