Overview

  • Product nameAnti-XRCC3 antibody
    See all XRCC3 primary antibodies
  • Description
    Rabbit polyclonal to XRCC3
  • Specificityab58467 detects endogenous levels of total XRCC1 protein.
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from internal sequence of human XRCC3.

  • Positive control
    • Extracts from HepG2 cells; human lung carcinoma tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab58467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/500.
WB 1/500 - 1/1000. Predicted molecular weight: 38 kDa.
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

Target

  • FunctionInvolved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and RAD51C.
  • Sequence similaritiesBelongs to the RecA family. RAD51 subfamily.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus. Cytoplasm. Cytoplasm > perinuclear region. Mitochondrion. Accumulates in discrete nuclear foci prior to DNA damage, and these foci persist throughout the time course of DNA repair.
  • Information by UniProt
  • Database links
  • Alternative names
    • CMM6 antibody
    • DNA repair protein XRCC3 antibody
    • RAD51 like antibody
    • X ray repair complementing defective repair in Chinese hamster antibody
    • X ray repair complementing defective repair in Chinese hamster cells 3 antibody
    • X ray repair cross complementing protein 3 antibody
    • X-ray repair cross-complementing protein 3 antibody
    • XRCC 3 antibody
    • XRCC3 antibody
    • XRCC3_HUMAN antibody
    see all

Anti-XRCC3 antibody images

  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ab58467 at a dilution of 1/50. Right hand image: treated with immunising peptide. Left hand image: untreated.
  • All lanes : Anti-XRCC3 antibody (ab58467) at 1/500 dilution

    Lane 1 : extracts from HepG2 cells treated with Adriamycin (0.5µM, 5 hours) with no immunising peptide
    Lane 2 : extracts from HepG2 cells treated with Adriamycin (0.5µM, 5 hours) with immunising peptide


    Predicted band size : 38 kDa
    Observed band size : 38 kDa
  • ab58467 at 1/500 dilution staining XRCC3 in COS7 cells by Immunofluorescence, in the absence or presence of the synthesized peptide.

References for Anti-XRCC3 antibody (ab58467)

ab58467 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"