FunctionInvolved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and RAD51C.
Sequence similaritiesBelongs to the RecA family. RAD51 subfamily.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Cytoplasm. Cytoplasm > perinuclear region. Mitochondrion. Accumulates in discrete nuclear foci prior to DNA damage, and these foci persist throughout the time course of DNA repair.
Immunohistochemical analysis of paraffin-embedded
human lung carcinoma tissue using ab58467 at a dilution of 1/50. Right hand image: treated with immunising peptide. Left hand image: untreated.
Western blot - XRCC3 antibody (ab58467)
All lanes : Anti-XRCC3 antibody (ab58467) at 1/500 dilution
Lane 1 : extracts from HepG2 cells treated with Adriamycin (0.5µM, 5 hours) with no immunising peptide Lane 2 : extracts from HepG2 cells treated with Adriamycin (0.5µM, 5 hours) with immunising peptide
Predicted band size : 38 kDa Observed band size : 38 kDa