Overview

  • Product nameAnti-XRCC3 antibody [XRCC3 10F1/6]
    See all XRCC3 primary antibodies
  • Description
    Mouse monoclonal [XRCC3 10F1/6] to XRCC3
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein (human) which has been his-tagged, overexpressed in E.coli and purified on a talon affinity column under denaturing conditions, followed by gel purification on a SDS-Page.

  • Positive control
    • Does not recognise XRCC3 in HeLa total extracts- suggest nuclear extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab20254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • FunctionInvolved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and RAD51C.
  • Sequence similaritiesBelongs to the RecA family. RAD51 subfamily.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus. Cytoplasm. Cytoplasm > perinuclear region. Mitochondrion. Accumulates in discrete nuclear foci prior to DNA damage, and these foci persist throughout the time course of DNA repair.
  • Information by UniProt
  • Database links
  • Alternative names
    • CMM6 antibody
    • DNA repair protein XRCC3 antibody
    • RAD51 like antibody
    • X ray repair complementing defective repair in Chinese hamster antibody
    • X ray repair complementing defective repair in Chinese hamster cells 3 antibody
    • X ray repair cross complementing protein 3 antibody
    • X-ray repair cross-complementing protein 3 antibody
    • XRCC 3 antibody
    • XRCC3 antibody
    • XRCC3_HUMAN antibody
    see all

Anti-XRCC3 antibody [XRCC3 10F1/6] images

  • Ab20254 staining human normal placenta. Staining is localized to nuclear compartment.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.


  • Predicted band size : 38 kDa

    See Abreview

References for Anti-XRCC3 antibody [XRCC3 10F1/6] (ab20254)

This product has been referenced in:
  • Li L  et al. Targeting poly(ADP-ribose) polymerase and the c-Myb-regulated DNA damage response pathway in castration-resistant prostate cancer. Sci Signal 7:ra47 (2014). Read more (PubMed: 24847116) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hep G2)
Loading amount 100000 cells
Specification Hep G2
Treatment 15 nM siRNA transfection for 72 hours
Gel Running Conditions Reduced Denaturing (4-12% SDS-PAGE)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Dr. Shijian Zhang

Verified customer

Submitted Jun 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 25 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 18 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"