Protein expressed in 293T cells transfected with Human XRCC4 expression vector.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC4; Human breast adenocarcinoma, colon, colon adenocarcinoma, kidney, lung, ovary, ovary adenocarcinoma, thyroid, thyroid carcinoma, endometrium, endometrium adenocarcinoma, prostate carcinoma or bladder carcinoma tissues.
General notesDilute in PBS (pH7.3) before use. Avoid repeated freeze-thaw cycles.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000. Predicted molecular weight: 38 kDa.
FunctionInvolved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the XRCC4 family.
Post-translational modificationsPhosphorylated by PRKDC. The phosphorylation seems not to be necessary for binding to DNA. Phosphorylation by CK2 promotes interaction with APTX. Monoubiquitinated. Sumoylation at Lys-210 is required for nuclear localization and recombination efficiency. Has no effect on ubiquitination.