Anti-XRCC4 antibody (ab58468)

Overview

  • Product name
  • Description
    Rabbit polyclonal to XRCC4
  • Specificity
    ab58468 detects endogenous levels of total XRCC4 protein.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from internal sequence of human XRCC4

  • Positive control
    • WB: Extracts from COLO205 cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab58468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 38 kDa).
ELISA 1/20000.

Target

  • Function
    Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the XRCC4 family.
  • Post-translational
    modifications
    Phosphorylated by PRKDC. The phosphorylation seems not to be necessary for binding to DNA. Phosphorylation by CK2 promotes interaction with APTX.
    Monoubiquitinated.
    Sumoylation at Lys-210 is required for nuclear localization and recombination efficiency. Has no effect on ubiquitination.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA double strand break repair and V(D)J recombination protein XRCC4 antibody
    • DNA repair protein XRCC4 antibody
    • SSMED antibody
    • X ray repair complementing defective repair in Chinese hamster cells 4 antibody
    • X ray repair cross complementing 4 antibody
    • X ray repair cross complementing protein 4 antibody
    • X-ray repair cross-complementing protein 4 antibody
    • XRCC 4 antibody
    • XRCC4 antibody
    • XRCC4_HUMAN antibody
    see all

Anti-XRCC4 antibody images

  • All lanes : Anti-XRCC4 antibody (ab58468) at 1/500 dilution

    Lane 1 : extracts from COLO205 cells with no immunising peptide
    Lane 2 : extracts from COLO205 cells with immunising peptide


    Predicted band size : 38 kDa

References for Anti-XRCC4 antibody (ab58468)

ab58468 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
ChIP
Sample
Human Cell lysate - whole cell (U2OS)
Negative control
No antibody (Mock) Mouse anti-rat IgG Primers distal to 2 DNA double-Strand breaks 2 Undamaged regions
Specification
U2OS
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Positive control
Anti XRCCA GTX70263 Primers proximal to 2 DNA double-Strand breaks
Username

Dr. Thomas Clouaire

Verified customer

Submitted Mar 24 2017



We have only tested this antibody in ELISA against the immunogenic peptide, so unfortunately we do not have any data from testing with cell lysates indicating the titer of XRCC4.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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