Anti-XRCC4 antibody (ab97351)


  • Product nameAnti-XRCC4 antibody
    See all XRCC4 primary antibodies
  • Description
    Rabbit polyclonal to XRCC4
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 266-279 of Human XRCC4 (NP_071801)

  • Positive control
    • 293T, A431, H1299, HeLaS3, HepG2, MOLT4, Raji and HeLa cells. A549 xenograft.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab97351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.
WB 1/500 - 1/3000. Predicted molecular weight: 38 kDa.
ICC/IF 1/100 - 1/200.


  • FunctionInvolved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the XRCC4 family.
  • Post-translational
    Phosphorylated by PRKDC. The phosphorylation seems not to be necessary for binding to DNA. Phosphorylation by CK2 promotes interaction with APTX.
    Sumoylation at Lys-210 is required for nuclear localization and recombination efficiency. Has no effect on ubiquitination.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA double strand break repair and V(D)J recombination protein XRCC4 antibody
    • DNA repair protein XRCC4 antibody
    • SSMED antibody
    • X ray repair complementing defective repair in Chinese hamster cells 4 antibody
    • X ray repair cross complementing 4 antibody
    • X ray repair cross complementing protein 4 antibody
    • X-ray repair cross-complementing protein 4 antibody
    • XRCC 4 antibody
    • XRCC4 antibody
    • XRCC4_HUMAN antibody
    see all

Anti-XRCC4 antibody images

  • All lanes : Anti-XRCC4 antibody (ab97351) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : HeLaS3 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 38 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97351 at 1/200 dilution. Lower image was also labelled with DNA probe.
  • Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ab97351 at 1/100 dilution.

References for Anti-XRCC4 antibody (ab97351)

ab97351 has not yet been referenced specifically in any publications.

Product Wall

Application ChIP
Sample Human Cell lysate - whole cell (U2OS)
Negative control Mock, untreated cells
Specification U2OS
Detection step Real-time PCR
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Positive control AsiSI induced DSB no1 and no2 (positive signal for anti XRCC4 ab145, RAD51 and gamma H2AX)

Dr. Thomas Clouaire

Verified customer

Submitted Dec 21 2015

Thank you very much for your call this morning and for your patience while I've reviewed the literature and other XRCC4 antibodies.

I found antibodies sold by other companies showingeither band size,two of which show bands at both 38 and 55 k...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (VSMC)
Specification VSMC
Fixative Formaldehyde
Permeabilization Yes - NP40
Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Dr. Andrew Cobb

Verified customer

Submitted Mar 09 2012

Application Western blot
Sample Mouse Tissue lysate - whole (mouse liver lysate)
Loading amount 10 µg
Specification mouse liver lysate
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Oct 26 2011