Anti-XRCC4 antibody [OTI4H9] (ab118008)

Overview

  • Product nameAnti-XRCC4 antibody [OTI4H9]
    See all XRCC4 primary antibodies
  • Description
    Mouse monoclonal [OTI4H9] to XRCC4
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Protein expressed in 293T cells transfected with Human XRCC4 expression vector.

  • Positive control
    • HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC4; Human breast adenocarcinoma, colon, colon adenocarcinoma, kidney, lung, ovary, ovary adenocarcinoma, thyroid, thyroid carcinoma, endometrium, endometrium adenocarcinoma, prostate carcinoma or bladder carcinoma tissues.
  • General notes

    Dilute in PBS (pH7.3) before use. Avoid repeated freeze-thaw cycles.

    The clone number has been updated from 4H9 to OTI4H9, both clone numbers name the same clone.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium azide
    Constituents: 1% BSA, 50% Glycerol, 48% PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab118008 is purified from Mouse ascite fluid by affinity chromatography.
  • ClonalityMonoclonal
  • Clone numberOTI4H9
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab118008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 38 kDa.
IHC-P 1/50.

Target

  • FunctionInvolved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the XRCC4 family.
  • Post-translational
    modifications
    Phosphorylated by PRKDC. The phosphorylation seems not to be necessary for binding to DNA. Phosphorylation by CK2 promotes interaction with APTX.
    Monoubiquitinated.
    Sumoylation at Lys-210 is required for nuclear localization and recombination efficiency. Has no effect on ubiquitination.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA double strand break repair and V(D)J recombination protein XRCC4 antibody
    • DNA repair protein XRCC4 antibody
    • SSMED antibody
    • X ray repair complementing defective repair in Chinese hamster cells 4 antibody
    • X ray repair cross complementing 4 antibody
    • X ray repair cross complementing protein 4 antibody
    • X-ray repair cross-complementing protein 4 antibody
    • XRCC 4 antibody
    • XRCC4 antibody
    • XRCC4_HUMAN antibody
    see all

Anti-XRCC4 antibody [OTI4H9] images

  • All lanes : Anti-XRCC4 antibody [OTI4H9] (ab118008) at 1/2000 dilution

    Lane 1 : HEK293T lysate transfected with pCMV6-ENTRY control cDNA for 48 hrs
    Lane 2 : HEK293T lysate transfected with pCMV6-ENTRY XRCC4 cDNA for 48 hrs

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 38 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC218029)
  • ab118008 at 1/50 dilution, staining XRCC4 in Paraffin- embedded Human breast adenocarcinoma tissue by Immunohistochemistry.
  • ab118008 at 1/50 dilution, staining XRCC4 in Paraffin- embedded Human Colon tissue by Immunohistochemistry.
  • ab118008 at 1/50 dilution, staining XRCC4 in Paraffin- embedded Human Colon adenocarcinoma tissue by Immunohistochemistry.
  • ab118008 at 1/50 dilution, staining XRCC4 in Paraffin- embedded Human Kidney tissue by Immunohistochemistry.
  • ab118008 at 1/50 dilution, staining XRCC4 in Paraffin- embedded Human Lung tissue by Immunohistochemistry.
  • ab118008 at 1/50 dilution, staining XRCC4 in Paraffin- embedded Human Ovary tissue by Immunohistochemistry.
  • ab118008 at 1/50 dilution, staining XRCC4 in Paraffin- embedded Human Ovary adenocarcinoma tissue by Immunohistochemistry.
  • ab118008 at 1/50 dilution, staining XRCC4 in Paraffin- embedded Human Thyroid tissue by Immunohistochemistry.
  • ab118008 at 1/50 dilution, staining XRCC4 in Paraffin- embedded Human Thyroid carcinoma tissue by Immunohistochemistry.
  • ab118008 at 1/50 dilution, staining XRCC4 in Paraffin- embedded Human endometrium tissue by Immunohistochemistry.
  • ab118008 at 1/50 dilution, staining XRCC4 in Paraffin- embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.
  • ab118008 at 1/50 dilution, staining XRCC4 in Paraffin- embedded Human Prostate carcinoma tissue by Immunohistochemistry.
  • ab118008 at 1/50 dilution, staining XRCC4 in Paraffin- embedded Human Bladder carcinoma tissue by Immunohistochemistry.

References for Anti-XRCC4 antibody [OTI4H9] (ab118008)

ab118008 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - whole cell (U2OS)
Negative control No antibody (Mock) Mouse anti-rat IgG Primers distal to 2 DNA double-Strand breaks 2 Undamaged regions
Specification U2OS
Detection step Real-time PCR
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Positive control Anti XRCCA GTX70263 Primers proximal to 2 DNA double-Strand breaks
Username

Dr. Thomas Clouaire

Verified customer

Submitted Mar 22 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"